CXADR/CAR (CXADR) Active Protein

Name: CXADR Active Protein; CXADR/CAR; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844913
Price: 310 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844913

Background: Coxsackie virus and adenovirus receptor (CXADR) also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CXADR/CAR, Active Protein

Full Product Name

Human CellExp CXADR / CAR, human recombinant

Product Gene Name

CXADR active protein

Product Synonym Names

CXADR; CAR; CAR4/6; HCAR

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with a C-terminal 6×His tag, has a calculated MW of 24.9 kDa expressed. The predicted N-terminus is Leu 20. Protein migrates as 35 kDa in reduced SDS-PAGE due to glycosylation.

OMIM

602621

3D Structure

ModBase 3D Structure for P78310

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 mug/mL and 100 muL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37 degree C.

Results

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CXADR active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzyme

Applications Tested/Suitable for CXADR active protein

SDS-PAGE

Other Notes

Small volumes of CXADR active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXADR purchase

MBS844913 is an active protein targeting CXADR. Also known as Human CellExp CXADR / CAR, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CXADR. It may not necessarily be applicable to this product.

NCBI GI #

333470703

NCBI GeneID

1525

NCBI Accession #

NP_001193992.1

UniProt Primary Accession #

P78310

UniProt Secondary Accession #

O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8; B7WPI3; D3YHP0

UniProt Related Accession #

P78310

GenBank Nucleotide #

NM_001207063.1

NCBI Official Full Name

coxsackievirus and adenovirus receptor isoform 2

NCBI Official Synonym Full Names

coxsackie virus and adenovirus receptor

NCBI Official Symbol

CXADR

NCBI Official Synonym Symbols

CAR; HCAR; CAR4/6

UniProt Protein Name

Coxsackievirus and adenovirus receptor

UniProt Synonym Protein Names

CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR

Protein Family

Coxsackievirus and adenovirus receptor

UniProt Gene Name

CXADR

UniProt Synonym Gene Names

CAR; CAR; hCAR

UniProt Entry Name

CXAR_HUMAN

NCBI Protein Information

coxsackievirus and adenovirus receptor

NCBI Summary for CXADR

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

UniProt Comments for CXADR

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Receptor, misc.; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; tight junction; protein complex; integral to plasma membrane; apicolateral plasma membrane; basolateral plasma membrane; extracellular region; acrosome; intercellular junction; lipid raft; nucleoplasm; growth cone; adherens junction; plasma membrane; cell junction; neuromuscular junction; filopodium

Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; neutrophil chemotaxis; mitochondrion organization and biogenesis; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; homotypic cell-cell adhesion; germ cell migration; blood coagulation; leukocyte migration; defense response to virus

Research Articles on CXADR

1. Suggest that distinct forms of CAR play different roles in the undifferentiated state and tight junction formation of human embryos and embryonic stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.