DuSP3 Active Protein

Name: DuSP3 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204275
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204275

DuSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DuSP3, Active Protein

Full Product Name

DuSP3, 1-185aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

DuSP3 active protein

Product Synonym Names

Dual specificity protein phosphatase 3; VHR

Sequence

MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

22.6 kDa (205aa), confirmed by MALDI-TOF

OMIM

L05147 mRNA

3D Structure

ModBase 3D Structure for P51452

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 1,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Product Categories/Family for DuSP3 active protein

Cancer

Applications Tested/Suitable for DuSP3 active protein

SDS-PAGE

Product References and Citations for DuSP3 active protein

Todd JL., et al. (1999) J Biol Chem. 274 (19):13271-80.; Keyse SM., et al. (1995) Biochim Biophys Acta. 1265 (2-3):152-60.

Other Notes

Small volumes of DuSP3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DuSP3 purchase

MBS204275 is an active protein targeting DuSP3. Also known as DuSP3, 1-185aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DuSP3. It may not necessarily be applicable to this product.

NCBI GI #

4758208

NCBI GeneID

1845

NCBI Accession #

NP_004081

UniProt Primary Accession #

P51452

UniProt Secondary Accession #

Q5U0J1; Q8IYJ9; D3DX45

UniProt Related Accession #

P51452

GenBank Nucleotide #

NM_004090.3

NCBI Official Full Name

dual specificity protein phosphatase 3

NCBI Official Synonym Full Names

dual specificity phosphatase 3

NCBI Official Symbol

DUSP3

NCBI Official Synonym Symbols

VHR

UniProt Protein Name

Dual specificity protein phosphatase 3

UniProt Synonym Protein Names

Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR

Protein Family

Dual specificity protein phosphatase

UniProt Gene Name

DUSP3

UniProt Synonym Gene Names

VHR; VHR

NCBI Protein Information

dual specificity protein phosphatase 3

NCBI Summary for DuSP3

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

UniProt Comments for DuSP3

Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.

Research Articles on DuSP3

1. Data indicated that regulating non-receptor tyrosine kinase FAK and cell migration is a newly revealed function of VHR/DUSP3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DuSP3 active protein

Reference Product	PubMed Publications
PTPRR active protein	>1 publications with DuSP3 and PTPRR
PTPN7 active protein	>1 publications with DuSP3 and PTPN7

Pathways associated with DuSP3 active protein

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
ERK/MAPK Targets Pathway antibodies	ERK/MAPK Targets Pathway Diagram
ERKs Are Inactivated Pathway antibodies	ERKs Are Inactivated Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
MAP Kinase Activation In TLR Cascade Pathway antibodies	MAP Kinase Activation In TLR Cascade Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Targets/ Nuclear Events Mediated By MAP Kinases Pathway antibodies	MAPK Targets/ Nuclear Events Mediated By MAP Kinases Pathway Diagram
MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies	MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram

Diseases associated with DuSP3 active protein

Disease Name	Pubmed Publications
Lung Diseases Antibodies	>9 publications with DuSP3 and Lung Diseases
Carcinoma Antibodies	>7 publications with DuSP3 and Carcinoma
Inflammation Antibodies	>6 publications with DuSP3 and Inflammation
Neoplasms, Experimental Antibodies	>4 publications with DuSP3 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>3 publications with DuSP3 and Lung Neoplasms
Carcinoma, Non-Small-Cell Lung Antibodies	>3 publications with DuSP3 and Carcinoma, Non-Small-Cell Lung
Immune System Diseases Antibodies	>2 publications with DuSP3 and Immune System Diseases
Carcinoma, Squamous Cell Antibodies	>2 publications with DuSP3 and Carcinoma, Squamous Cell
Heart Diseases Antibodies	>2 publications with DuSP3 and Heart Diseases
Nervous System Diseases Antibodies	>2 publications with DuSP3 and Nervous System Diseases

Organs/Tissues associated with DuSP3 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>16 publications with DuSP3 and Blood
Lung Antibodies	>7 publications with DuSP3 and Lung
Brain Antibodies	>4 publications with DuSP3 and Brain
Embryonic Tissue Antibodies	>2 publications with DuSP3 and Embryonic Tissue
Cervix Antibodies	>2 publications with DuSP3 and Cervix
Bone Marrow Antibodies	>2 publications with DuSP3 and Bone Marrow
Nerve Antibodies	>2 publications with DuSP3 and Nerve
Bone Antibodies	>2 publications with DuSP3 and Bone
Heart Antibodies	>2 publications with DuSP3 and Heart
Liver Antibodies	>1 publications with DuSP3 and Liver