EphB6 Active Protein

Name: EphB6 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS8123143
Price: 585 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8123143

Background: Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual… More details

$585 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EphB6, Active Protein

Full Product Name

EphB6 Protein, Mouse, Recombinant (His Tag)

Product Gene Name

EphB6 active protein

Product Synonym Names

Mouse EphB6 Protein (His Tag); Cekl Protein, Mouse; Mep Protein, Mouse; EPH receptor B6

Sequence

Met1-Ser58 7

Host

HEK293 Cells

Form/Format

Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Purity/Purification

>85% as determined by SDS-PAGE

Preparation and Storage

Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

Molecular Weight

110,107 Da

Chromosome Location

Chromosome: 6; NC_000072.6 (41605042..41620507). Location: 6 B2.1; 6

3D Structure

ModBase 3D Structure for O08644

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Mouse

Endotoxin

<1.0EU per ug of the protein as determined by the LAL method

Predicted N Terminal

Leu 33

Tag

C-His

Protein Construction

A DNA sequence encoding the mouse EPHB6 (O08644-1)(Met1-Ser587) was expressed with a C-terminal polyhistidine tag.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Activity

1. Immobilized mouse EPHB6-His at 10ug/ml (100uL/well) can bind mouse EFNB1-Fc. The EC50 of mouse EFNB1-Fc is 0.12-0.28ug/ml.
2. Immobilized mouse EPHB6-His at 10ug/ml (100uL/well) can bind mouse EFNB2-Fc. The EC50 of mouse EFNB2-Fc is 0.04-0.08ug/ml.

Product Categories/Family for EphB6 active protein

Actin Dynamics Signaling Pathway

Product References and Citations for EphB6 active protein

Munthe E, et al. (2000)Ephrin-B2 is a candidate ligand for the Eph receptor, EphB6. FEBS Lett. 466(1): 169-74.Matsuoka H, et al. (2005) Biphasic functions of the kinase-defective Ephb6 receptor in cell adhesion and migration. J Biol Chem. 280(32): 29355-63.Truitt L, et al. (2010) The EphB6 receptor cooperates with c-Cbl to regulate the behavior of breast cancer cells. Cancer Res. 70(3): 1141-53.

Other Notes

Small volumes of EphB6 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EphB6 purchase

MBS8123143 is an active protein targeting EphB6. Also known as EphB6 Protein, Mouse, Recombinant (His Tag). Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for EphB6. It may not necessarily be applicable to this product.

NCBI GI #

226437671

NCBI GeneID

13848

NCBI Accession #

NP_001139823.1

UniProt Primary Accession #

O08644

UniProt Secondary Accession #

Q3TQ77; Q8BN76; Q8K0A9

UniProt Related Accession #

O08644

GenBank Nucleotide #

NM_001146351.1

NCBI Official Full Name

ephrin type-B receptor 6

NCBI Official Synonym Full Names

Eph receptor B6

NCBI Official Symbol

Ephb6

NCBI Official Synonym Symbols

Mep; Cekl

UniProt Protein Name

Ephrin type-B receptor 6

UniProt Synonym Protein Names

MEP; Tyrosine-protein kinase-defective receptor EPH-6

Protein Family

Ephrin type-B receptor

UniProt Gene Name

Ephb6

UniProt Synonym Gene Names

Cekl

UniProt Entry Name

EPHB6_MOUSE

NCBI Protein Information

ephrin type-B receptor 6; ephrin type A receptor; chicken eph/elk receptor-like protein; tyrosine-protein kinase-defective receptor EPH-6

UniProt Comments for EphB6

Function: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2

By similarity.

Subunit structure: Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner

By similarity.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted.

Tissue specificity: High level in thymus, and brain. Very low levels of expression in kidney, lung, liver, bone marrow, skeletal muscle, spleen from 2 week old and adult mice, heart, testes and embryonic stem cells. Ref.1

Domain: The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2

By similarity.

Post-translational modification: Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.

Research Articles on EphB6

1. Ephs and Efns, in concert with testosterone, play a critical role in regulating small artery contractility and blood pressure.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with EphB6 active protein

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Diseases associated with EphB6 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>39 publications with EphB6 and Neoplasms
Breast Neoplasms Antibodies	>11 publications with EphB6 and Breast Neoplasms
Nervous System Diseases Antibodies	>6 publications with EphB6 and Nervous System Diseases
Brain Diseases Antibodies	>5 publications with EphB6 and Brain Diseases
Neoplasms, Experimental Antibodies	>4 publications with EphB6 and Neoplasms, Experimental
Liver Neoplasms Antibodies	>3 publications with EphB6 and Liver Neoplasms
Cardiovascular Diseases Antibodies	>2 publications with EphB6 and Cardiovascular Diseases
Atherosclerosis Antibodies	>1 publications with EphB6 and Atherosclerosis
Hypertension Antibodies	>1 publications with EphB6 and Hypertension
Weight Loss Antibodies	>1 publications with EphB6 and Weight Loss

Organs/Tissues associated with EphB6 active protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>12 publications with EphB6 and Lung
Brain Antibodies	>10 publications with EphB6 and Brain
Thymus Antibodies	>7 publications with EphB6 and Thymus
Bone Antibodies	>6 publications with EphB6 and Bone
Embryonic Tissue Antibodies	>5 publications with EphB6 and Embryonic Tissue
Skin Antibodies	>2 publications with EphB6 and Skin
Intestine Antibodies	>2 publications with EphB6 and Intestine
Eye Antibodies	>1 publications with EphB6 and Eye
Mammary Gland Antibodies	>1 publications with EphB6 and Mammary Gland