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Fibroblast Growth Factor-Basic Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546095

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Fibroblast Growth Factor-basic, Active Protein
Full Product Name
Recombinant Rat Fibroblast Growth Factor-basic (rRa bFGF)
Product Synonym Names
Fibroblast Growth Factor-basic; rat FGF-basic; Rat Fibroblast Growth Factor-basic
Host
E Coli
Form/Format
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
>98% by SDS-PAGE and HPLC analyses
Preparation and Storage
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Molecular Weight Information
Approximately 16 kDa, a single non-glycosylated polypeptide chain containing 151 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells is <= 0.2 ng/ml,corresponding to a specific activity of >=5 x 106 units/mg.
Endotoxin Level
Less than 1EU/mg of rRabFGF as determined by LAL method
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of Fibroblast Growth Factor-basic active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor-basic purchase

MBS546095 is an active protein targeting Fibroblast Growth Factor-basic. Also known as Recombinant Rat Fibroblast Growth Factor-basic (rRa bFGF). Host: E Coli.
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