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glk Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206264

glk belongs to the bacterial glucokinase family. This protein is not highly important in E Coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E Coli glk protein, fused to His-tag at N-terminus, was… More details

$215 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
glk, Active Protein
Full Product Name
glk, 1-321aa, E Coli, His tagged, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
Glucokinase; ECK2384; JW2385
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol.
Purity/Purification
> 95% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
37.1kDa (344aa), confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P0A6V8
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
E Coli
Tag Information
His-tag
Biological Activity
Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.

Product Categories/Family for glk active protein

Applications Tested/Suitable for glk active protein
SDS-PAGE

SDS-Page of glk active protein

glk Active Protein – Cat# MBS206264

Product References and Citations for glk active protein

Kawai S, Mukai T, et al. (2005) J. Biosci. Bioeng. 99 (4): 320-30.

Other Notes

Small volumes of glk active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for glk purchase

MBS206264 is an active protein targeting glk. Also known as glk, 1-321aa, E Coli, His tagged, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for glk. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
glucokinase
NCBI Official Symbol
glk
NCBI Official Synonym Symbols
ECK2384; JW2385
UniProt Protein Name
Glucokinase
UniProt Synonym Protein Names
Glucose kinase
Protein Family
UniProt Gene Name
glk
NCBI Protein Information
glucokinase

NCBI Summary for glk

The cytoplasmic glucokinase is not required for growth on glucose as the carbon source, because glucose is phosphorylated during transport by the PTS system. [More information is available at EcoCyc: EG12957].

UniProt Comments for glk

Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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