GOT2 Active Protein

Name: GOT2 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844883
Price: 460 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844883

Background: GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

GOT2, Active Protein

Full Product Name

GOT2, human recombinant

Product Gene Name

GOT2 active protein

Product Synonym Names

Aspartate aminotransferase 2; mitochondrial; KAT4; KATIV; mitAAT

Sequence

MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

Host

E Coli

Form/Format

0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 150 mM NaCl and 1 mM DTT.
Appearance: Liquid

Purity/Purification

>=95%

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

48.4 kDa (433 aa, 1-413 aa + His Tag)

3D Structure

ModBase 3D Structure for P05202

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 44 unit/mg, in which one unit will convert 1.0 umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25 degree C.

Results

Specific activity is > 44 unit/mg

Unit Definition

One unit will convert 1.0 umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25 degree C.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for GOT2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for GOT2 active protein

SDS-PAGE

Other Notes

Small volumes of GOT2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT2 purchase

MBS844883 is an active protein targeting GOT2. Also known as GOT2, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT2. It may not necessarily be applicable to this product.

NCBI GI #

6754036

NCBI GeneID

14719

NCBI Accession #

NP_034455.1

UniProt Primary Accession #

P05202

UniProt Secondary Accession #

O09188; Q3TIP6; Q3UD91; Q5HZH5

UniProt Related Accession #

P05202

GenBank Nucleotide #

NM_010325.2

NCBI Official Full Name

aspartate aminotransferase, mitochondrial

NCBI Official Synonym Full Names

glutamatic-oxaloacetic transaminase 2, mitochondrial

NCBI Official Symbol

Got2

NCBI Official Synonym Symbols

Got-2; mAspAT; FABP-pm; AL022787

UniProt Protein Name

Aspartate aminotransferase, mitochondrial

UniProt Synonym Protein Names

Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A

UniProt Gene Name

Got2

UniProt Synonym Gene Names

Got-2; mAspAT; FABP-1; FABPpm

UniProt Entry Name

AATM_MOUSE

NCBI Protein Information

aspartate aminotransferase, mitochondrial

UniProt Comments for GOT2

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 2.6.1.1; Amino Acid Metabolism - cysteine and methionine; Transferase; Amino Acid Metabolism - phenylalanine; Mitochondrial; Amino Acid Metabolism - tyrosine; EC 2.6.1.7

Cellular Component: protein complex; cell surface; mitochondrion; membrane; mitochondrial inner membrane; plasma membrane; perikaryon; myelin sheath

Molecular Function: transferase activity; amino acid binding; protein homodimerization activity; enzyme binding; kynurenine-oxoglutarate transaminase activity; carboxylic acid binding; aspartate transaminase activity; phospholipid binding; transaminase activity; catalytic activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; dicarboxylic acid metabolic process; response to ethanol; oxaloacetate metabolic process; transport; glutamate catabolic process to aspartate; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; glutamate catabolic process to 2-oxoglutarate; lipid transport

Research Articles on GOT2

1. Biochemical and structural basis towards understanding the overall physiological role of mouse mitochondrial aspartate aminotransferase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.