Human IL21 Active Protein – Cat# MBS4159677

IL21 Human Active Protein

Name: IL21 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS4159677
Price: 475 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4159677

IL-21 stimulates proliferation of B cell stimulated by crosslinking of the CD40 antigen. It inhibits proliferation stimulated by IL-4 plus anti-IgM. IL-21 augments stimulation of the proliferation of naive CD45RA(+) but not memory CD45R0(+) T cells mediated… More details

$475 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL21, Active Protein

Full Product Name

Recombinant Human IL21

Product Gene Name

IL21 active protein

Product Synonym Names

IL-21; Za11; Recombinant Human Interleukin-21 (IL21)

Host

E Coli

Form/Format

Lyophilized from a 0.2 um filtered PBS solution pH 7.5.

Purity/Purification

>95% as determined by SDS-PAGE

Preparation and Storage

The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Molecular Weight

15.0 kDa

OMIM

605384

3D Structure

ModBase 3D Structure for Q9HBE4

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Bioactivity

The ED(50) was determined by the dose-dependent proliferation of HuT-78 Cells and was found to be <0.05ng/mL.

Endotoxin Level

<1.0 EU/ug of recombinant protein as determined by the LAL method.

Reconstitution

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Other Notes

Small volumes of IL21 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL21 purchase

MBS4159677 is an active protein targeting IL21. Also known as Recombinant Human IL21. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL21. It may not necessarily be applicable to this product.

NCBI GI #

333033767

NCBI GeneID

59067

NCBI Accession #

NP_001193935.1

UniProt Primary Accession #

Q9HBE4

UniProt Related Accession #

Q9HBE4

GenBank Nucleotide #

NP_001193935.1

NCBI Official Full Name

interleukin-21 isoform 2

NCBI Official Synonym Full Names

interleukin 21

NCBI Official Symbol

IL21

NCBI Official Synonym Symbols

Za11; IL-21; CVID11

UniProt Protein Name

Interleukin-21

UniProt Synonym Protein Names

Za11

Protein Family

Interleukin

UniProt Gene Name

IL21

UniProt Synonym Gene Names

IL-21

UniProt Entry Name

IL21_HUMAN

NCBI Protein Information

interleukin-21

NCBI Summary for IL21

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for IL21

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Secreted, signal peptide; Secreted; Cytokine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; signal transduction; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tissue remodeling; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response

Disease: Immunodeficiency, Common Variable, 11

Research Articles on IL21

1. This study suggests, for the first time that IL-21 enhances TLR2/4-mediated cytokine production in human monocytic THP-1 cells by activating the STAT3, PI3K/Akt and p38 MAPK signalling pathways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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