Human IL3 Active Protein – Cat# MBS546113

Interleukin-3 (IL3) Human Active Protein

Name: IL3 Active Protein; Interleukin-3; Human; MyBioSource
Brand: MyBioSource
SKU: MBS546113
Price: 290 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546113

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Interleukin-3 (IL3), Active Protein

Full Product Name

Recombinant Human Interleukin-3 (rHu IL-3)

Product Gene Name

IL3 active protein

Product Synonym Names

Interleukin-3; human IL-3; Human Interleukin-3

Host

E Coli

Form/Format

Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

>97% by SDS-PAGE and HPLC analyses.

Preparation and Storage

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Molecular Weight

17,233 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (131396347..131398896). Location: 5q31.1

OMIM

147740

3D Structure

ModBase 3D Structure for P08700

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Molecular Weight Information

Approximately 15 kDa, a single non-glycosylated polypeptide chain containing 130 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 107 IU/mg.

Endotoxin Level

Less than 1EU/mg of rHuIL-3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of IL3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL3 purchase

MBS546113 is an active protein targeting IL3. Also known as Recombinant Human Interleukin-3 (rHu IL-3). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL3. It may not necessarily be applicable to this product.

NCBI GI #

28416915

NCBI GeneID

3562

NCBI Accession #

NP_000579.2

UniProt Primary Accession #

P08700

UniProt Secondary Accession #

Q6GS87

UniProt Related Accession #

P08700

GenBank Nucleotide #

NM_000588.3

NCBI Official Full Name

interleukin-3

NCBI Official Synonym Full Names

interleukin 3 (colony-stimulating factor, multiple)

NCBI Official Symbol

IL3

NCBI Official Synonym Symbols

IL-3; MCGF; MULTI-CSF

UniProt Protein Name

Interleukin-3

UniProt Synonym Protein Names

Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

Protein Family

Interleukin

UniProt Gene Name

IL3

UniProt Synonym Gene Names

IL-3; MCGF

UniProt Entry Name

IL3_HUMAN

NCBI Protein Information

interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor

NCBI Summary for IL3

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

UniProt Comments for IL3

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Oncoprotein; Secreted; Cell cycle regulation; Cytokine; Secreted, signal peptide; Apoptosis

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity

Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; cell-cell signaling; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication

Research Articles on IL3

1. Genetic variation in the IL3 promoter affects human brain volume by regulating proliferation and survival of neural progenitors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL3 active protein

Reference Product	PubMed Publications
IL2 active protein	>80 publications with IL3 and IL2
IL5 active protein	>49 publications with IL3 and IL5
JAK2 active protein	>13 publications with IL3 and JAK2
CSF2RB active protein	>10 publications with IL3 and CSF2RB
IL7 active protein	>9 publications with IL3 and IL7
FOS active protein	>7 publications with IL3 and FOS
IL3RA active protein	>7 publications with IL3 and IL3RA
GRB2 active protein	>2 publications with IL3 and GRB2
PIK3CA active protein	>1 publications with IL3 and PIK3CA

Pathways associated with IL3 active protein

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with IL3 active protein

Disease Name	Pubmed Publications
Hematologic Diseases Antibodies	>50 publications with IL3 and Hematologic Diseases
Bone Marrow Diseases Antibodies	>38 publications with IL3 and Bone Marrow Diseases
Necrosis Antibodies	>27 publications with IL3 and Necrosis
Disease Models, Animal Antibodies	>25 publications with IL3 and Disease Models, Animal
Lung Diseases Antibodies	>20 publications with IL3 and Lung Diseases
Inflammation Antibodies	>18 publications with IL3 and Inflammation
Skin Diseases Antibodies	>16 publications with IL3 and Skin Diseases
Asthma Antibodies	>15 publications with IL3 and Asthma
Anemia Antibodies	>12 publications with IL3 and Anemia
Nervous System Diseases Antibodies	>9 publications with IL3 and Nervous System Diseases

Organs/Tissues associated with IL3 active protein

Organ/Tissue Name	Pubmed Publications
Mixed Antibodies	>11 publications with IL3 and Mixed