1ug/lane of Recombinant Mouse Klotho beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 135kD.

Klotho Beta (KLB) Active Protein

Name: KLB Active Protein; Klotho Beta; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS635824
Price: 1220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635824

Klotho b, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue.1 Like Klotho, Klotho b facilitates binding between FGF19 subfamily members and their receptors… More details

$1220 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Klotho beta (KLB), Active Protein

Full Product Name

Klotho beta, Recombinant, Mouse (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

Product Gene Name

KLB active protein

Form/Format

Supplied as a liquid in PBS, 0.1 mM EDTA, 50% glycerol, pH 6.8.

Purity/Purification

Purified
90%, as determined by SDS-PAGE and visualized by silver stain.

Specificity

Recognizes mouse Klotho beta. species sequence homology: Human 79%, bovine 79%, canine 80% and rat Klotho beta 67%.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

110.2kD. As a result of glycosylation, the recombinant mouse Klotho Beta migrates as an approximately 140kD protein in SDS-PAGE under reducing conditions.

Chromosome Location

Chromosome: 4; NC_000004.11 (39408473..39453153). Location: 4p14

OMIM

611135

3D Structure

ModBase 3D Structure for Q86Z14

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Immunogen

NS0-derived recombinant mouse Klotho.

Biological Activity

Measured by its ability to stimulate proliferation of NIH 3T3 cells. The ED50 for this effect is typically 0.75-3ug/ml in the presence of 2ug/ml of rhFGF-21

Endotoxin Level

1EU/ug of the protein as determined by the LAL method.

Hybridoma

A DNA sequence encoding the extracellular domain of mouse Klotho Beta was fused to a polyhistdine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.

Product Categories/Family for KLB active protein

Molecular Biology; MB-Enzymes; Glycosidase

Other Notes

Small volumes of KLB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLB purchase

MBS635824 is an active protein targeting KLB. Also known as Klotho beta, Recombinant, Mouse (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein).

Additional Details

NCBI/Uniprot data below describe general gene information for KLB. It may not necessarily be applicable to this product.

NCBI GI #

119613331

NCBI GeneID

152831

NCBI Accession #

EAW92925.1

UniProt Primary Accession #

Q86Z14

UniProt Secondary Accession #

Q2M3K8

UniProt Related Accession #

Q86Z14

NCBI Official Full Name

klotho beta

NCBI Official Synonym Full Names

klotho beta

NCBI Official Symbol

KLB

NCBI Official Synonym Symbols

BKL

UniProt Protein Name

Beta-klotho

UniProt Synonym Protein Names

Klotho beta-like protein

Protein Family

Beta-klotho

UniProt Gene Name

KLB

UniProt Synonym Gene Names

BKL; BetaKlotho

UniProt Entry Name

KLOTB_HUMAN

NCBI Protein Information

beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein

UniProt Comments for KLB

Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; fibroblast growth factor binding; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response

Research Articles on KLB

1. betaKlotho suppresses tumor growth in hepatocellular carcinoma by regulating Akt/GSK-3beta/cyclin D1 signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KLB active protein

Reference Product	PubMed Publications
FGF21 active protein	>23 publications with KLB and FGF21
FGF19 active protein	>18 publications with KLB and FGF19
FGFR4 active protein	>14 publications with KLB and FGFR4
FGFR1 active protein	>12 publications with KLB and FGFR1
FGF23 active protein	>5 publications with KLB and FGF23
NR0B2 active protein	>1 publications with KLB and NR0B2
CYP8B1 active protein	>1 publications with KLB and CYP8B1

Pathways associated with KLB active protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies	Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies	Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
Downstream Signaling Of Activated FGFR Pathway antibodies	Downstream Signaling Of Activated FGFR Pathway Diagram
FGF Signaling Pathway antibodies	FGF Signaling Pathway Diagram
FGFR Ligand Binding And Activation Pathway antibodies	FGFR Ligand Binding And Activation Pathway Diagram

Diseases associated with KLB active protein

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>5 publications with KLB and Liver Diseases
Fatty Liver Antibodies	>5 publications with KLB and Fatty Liver
Neoplasms Antibodies	>4 publications with KLB and Neoplasms
Disease Models, Animal Antibodies	>4 publications with KLB and Disease Models, Animal
Weight Loss Antibodies	>2 publications with KLB and Weight Loss
Carcinoma, Hepatocellular Antibodies	>2 publications with KLB and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>2 publications with KLB and Liver Neoplasms
Inflammation Antibodies	>2 publications with KLB and Inflammation
Cardiovascular Diseases Antibodies	>1 publications with KLB and Cardiovascular Diseases
Necrosis Antibodies	>1 publications with KLB and Necrosis

Organs/Tissues associated with KLB active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>24 publications with KLB and Liver
Adipose Tissue Antibodies	>13 publications with KLB and Adipose Tissue
Brain Antibodies	>2 publications with KLB and Brain
Eye Antibodies	>1 publications with KLB and Eye