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LDHA Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8123490

Background: LDHA (Lactate Dehydrogenase A) is a Protein Coding gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA, a key enzyme regulating aerobic… More details

$415 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LDHA, Active Protein
Full Product Name
LDHA Protein, Rat, Recombinant (His Tag)
Product Gene Name
LDHA active protein
Product Synonym Names
Rat LDH-A/LDHA Protein (His Tag); lactate dehydrogenase A
Sequence
Met1-Phe33 2
Host
E Coli
Form/Format
Lyophilized from sterile 50mM Tris, 50mM NaCl. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Purity/Purification
>95% as determined by SDS-PAGE
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Molecular Weight
36,451 Da
3D Structure
ModBase 3D Structure for P04642
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Rat
Activity
Measured by its ability to reduce pyruvate to lactate. The specific activity is >50,000pmoles/min/ug.
Predicted N Terminal
Met
Tag
N-His
Protein Construction
A DNA sequence encoding the rat Ldha (NP_058721.1)(Met1-Phe332) was expressed with a polyhistidine tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-Page of LDHA active protein

LDHA Active Protein – Cat# MBS8123490

Other Notes

Small volumes of LDHA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDHA purchase

MBS8123490 is an active protein targeting LDHA. Also known as LDHA Protein, Rat, Recombinant (His Tag). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHA. It may not necessarily be applicable to this product.

NCBI GI #
8393706
NCBI GeneID
24533
NCBI Accession #
NP_058721.1
UniProt Primary Accession #
P04642
UniProt Related Accession #
P04642
GenBank Nucleotide #
NM_017025.1
NCBI Official Full Name
L-lactate dehydrogenase A chain
NCBI Official Synonym Full Names
lactate dehydrogenase A
NCBI Official Symbol
Ldha
NCBI Official Synonym Symbols
CDK1; Ldh1
UniProt Protein Name
L-lactate dehydrogenase A chain
UniProt Synonym Protein Names
LDH muscle subunit; LDH-M
Protein Family
L-lactate dehydrogenase
UniProt Gene Name
Ldha
UniProt Synonym Gene Names
Ldh-1; Ldh1; LDH-A; LDH-M
NCBI Protein Information
L-lactate dehydrogenase A chain

NCBI Summary for LDHA

mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]

UniProt Comments for LDHA

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytosol; membrane; mitochondrion; nucleus

Molecular Function: identical protein binding; kinase binding; L-lactate dehydrogenase activity; lactate dehydrogenase activity; NAD binding; protein binding

Biological Process: cellular response to extracellular stimulus; glucose catabolic process to lactate via pyruvate; lactate metabolic process; NAD metabolic process; positive regulation of apoptosis; post-embryonic organ development; response to cAMP; response to drug; response to estrogen stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to nutrient; response to organic cyclic substance; substantia nigra development
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Research Articles on LDHA

1. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with LDHA active protein

 Products by Pathway  Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram

Diseases associated with LDHA active protein

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >22 publications with LDHA and Adenocarcinoma
 Breast Neoplasms Antibodies  >14 publications with LDHA and Breast Neoplasms
 Liver Diseases Antibodies  >13 publications with LDHA and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >12 publications with LDHA and Carcinoma, Hepatocellular
 Neoplasm Metastasis Antibodies  >10 publications with LDHA and Neoplasm Metastasis
 Liver Neoplasms Antibodies  >10 publications with LDHA and Liver Neoplasms
 Neoplasm Invasiveness Antibodies  >9 publications with LDHA and Neoplasm Invasiveness
 Neoplasms, Experimental Antibodies  >9 publications with LDHA and Neoplasms, Experimental
 Kidney Diseases Antibodies  >8 publications with LDHA and Kidney Diseases
 Cardiovascular Diseases Antibodies  >8 publications with LDHA and Cardiovascular Diseases

Organs/Tissues associated with LDHA active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >27 publications with LDHA and Liver
 Muscle Antibodies  >25 publications with LDHA and Muscle
 Brain Antibodies  >23 publications with LDHA and Brain
 Testis Antibodies  >20 publications with LDHA and Testis
 Pancreas Antibodies  >14 publications with LDHA and Pancreas
 Kidney Antibodies  >12 publications with LDHA and Kidney
 Lung Antibodies  >12 publications with LDHA and Lung
 Heart Antibodies  >11 publications with LDHA and Heart
 Embryonic Tissue Antibodies  >9 publications with LDHA and Embryonic Tissue
 Ovary Antibodies  >9 publications with LDHA and Ovary
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