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LDLR/LDL Receptor (LDLR) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8122672

Background: LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated… More details

$310 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LDLR/LDL Receptor, Active Protein
Full Product Name
LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag)
Product Gene Name
LDLR active protein
Product Synonym Names
Mouse LDLR/LDL Receptor Protein (His Tag); Hlb301 Protein, Mouse; low density lipoprotein receptor
Sequence
Met1-Arg79 0
Host
HEK293 Cells
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Purity/Purification
>90% as determined by SDS-PAGE
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Molecular Weight
95,135 Da
Chromosome Location
Chromosome: 9; NC_000075.6 (21723576..21749919). Location: 9 A3; 9 5.0 cM
3D Structure
ModBase 3D Structure for P35951
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat PCSK9 at 10ug/ml (100uL/well) can bind biotinylated recombinant mouse LDLR. The EC50 of biotinylated mouse LDLR is 0.173ug/ml.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Ala 22
Tag
C-His
Protein Construction
A DNA sequence encoding the extracellular domain of mouse LDLR (NP_034830.2) precursor (Met 1-Arg 790) with substitution of Val 23 and Gly 27 by Ala 23 and Cys 27 respectively was expressed with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-Page of LDLR active protein

LDLR Active Protein – Cat# MBS8122672

Testing Data of LDLR active protein

Measured by its binding ability in a functional ELISA. Immobilized Rat PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant mouse LDLR. The EC50 of biotinylated mouse LDLR is 0.173 ug/ml.
LDLR Active Protein, Testing Data – Cat# MBS8122672

Product References and Citations for LDLR active protein

Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.

Other Notes

Small volumes of LDLR active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDLR purchase

MBS8122672 is an active protein targeting LDLR. Also known as LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag). Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for LDLR. It may not necessarily be applicable to this product.

NCBI GI #
113195700
NCBI GeneID
16835
NCBI Accession #
NP_034830.2
UniProt Primary Accession #
P35951
UniProt Related Accession #
P35951
GenBank Nucleotide #
NM_010700.3
NCBI Official Full Name
low-density lipoprotein receptor isoform 1
NCBI Official Synonym Full Names
low density lipoprotein receptor
NCBI Official Symbol
Ldlr
NCBI Official Synonym Symbols
Hlb301
UniProt Protein Name
Low-density lipoprotein receptor
Protein Family
Low-density lipoprotein receptor
UniProt Gene Name
Ldlr
UniProt Synonym Gene Names
LDL receptor
UniProt Entry Name
LDLR_MOUSE
NCBI Protein Information
low-density lipoprotein receptor; LDL receptor

UniProt Comments for LDLR

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell surface; Membrane protein, integral

Cellular Component: apical part of cell; basolateral plasma membrane; caveola; cell surface; coated pit; early endosome; endosome; external side of plasma membrane; Golgi apparatus; late endosome; lysosome; membrane; plasma membrane; receptor complex; recycling endosome membrane

Molecular Function: glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; very-low-density lipoprotein receptor activity

Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; lipid metabolic process; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; positive regulation of inflammatory response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LDLR active protein

 Reference Product  PubMed Publications
 Dab1 active protein  >3 publications with LDLR and Dab1
 Snx17 active protein  >2 publications with LDLR and Snx17

Pathways associated with LDLR active protein

 Products by Pathway  Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 LDL-mediated Lipid Transport Pathway antibodies  LDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram

Diseases associated with LDLR active protein

 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >829 publications with LDLR and Atherosclerosis
 Cardiovascular Diseases Antibodies  >742 publications with LDLR and Cardiovascular Diseases
 Hypercholesterolemia Antibodies  >669 publications with LDLR and Hypercholesterolemia
 Dyslipidemias Antibodies  >660 publications with LDLR and Dyslipidemias
 Hyperlipidemias Antibodies  >621 publications with LDLR and Hyperlipidemias
 HYPERCHOLESTEROLEMIA, FAMILIAL Antibodies  >449 publications with LDLR and HYPERCHOLESTEROLEMIA, FAMILIAL
 Hyperlipoproteinemia Type II Antibodies  >321 publications with LDLR and Hyperlipoproteinemia Type II
 Coronary Artery Disease Antibodies  >205 publications with LDLR and Coronary Artery Disease
 Coronary Disease Antibodies  >204 publications with LDLR and Coronary Disease
 Inflammation Antibodies  >183 publications with LDLR and Inflammation

Organs/Tissues associated with LDLR active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >918 publications with LDLR and Blood
 Liver Antibodies  >504 publications with LDLR and Liver
 Heart Antibodies  >228 publications with LDLR and Heart
 Bone Antibodies  >161 publications with LDLR and Bone
 Muscle Antibodies  >154 publications with LDLR and Muscle
 Bone Marrow Antibodies  >137 publications with LDLR and Bone Marrow
 Brain Antibodies  >115 publications with LDLR and Brain
 Kidney Antibodies  >60 publications with LDLR and Kidney
 Ovary Antibodies  >54 publications with LDLR and Ovary
 Intestine Antibodies  >42 publications with LDLR and Intestine
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