MGMT, Western Blot Control (MGMT) Active Protein

Name: MGMT Active Protein; MGMT, Western Blot Control; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS636503
Price: 720 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636503

MGMT (O6-methylguanine-DNA methyltransferase). Many tumor promoting/causing agents (BCNU, CCNU, DTIC, procarbazine, temozolomide, etc) generate lethal cross-links at the O6-alkylguanine position in DNA. MGMT removes alkyl adducts from the O6-position of… More details

$720 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MGMT, Western Blot Control, Active Protein

Full Product Name

MGMT, Recombinant, Human, Western Blot Control (O6-Methylguanine DNA Methyltransferase)

Product Gene Name

Mgmt active protein

Form/Format

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Purity/Purification

Purified
Purified

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

22,244 Da

Chromosome Location

Chromosome: 1; NC_005100.3 (216161435..216388310). Location: 1q41

3D Structure

ModBase 3D Structure for P24528

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Biologically inactive

Product Categories/Family for Mgmt active protein

Molecular Biology; MB-Enzymes; Methyltransferase

Applications Tested/Suitable for Mgmt active protein

Western Blot (WB)

Application Notes for Mgmt active protein

Suitable for use in Western Blot.
Dilution: Western Blot: Heat once and load 10ul/lane and visualize with M3884-91C & M3884-91E.

Other Notes

Small volumes of Mgmt active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mgmt purchase

MBS636503 is an active protein targeting Mgmt. Also known as MGMT, Recombinant, Human, Western Blot Control (O6-Methylguanine DNA Methyltransferase).

Additional Details

NCBI/Uniprot data below describe general gene information for Mgmt. It may not necessarily be applicable to this product.

NCBI GI #

127071

NCBI GeneID

25332

NCBI Accession #

P24528.3

UniProt Primary Accession #

P24528

UniProt Related Accession #

P24528

NCBI Official Full Name

Methylated-DNA--protein-cysteine methyltransferase

NCBI Official Synonym Full Names

O-6-methylguanine-DNA methyltransferase

NCBI Official Symbol

Mgmt

UniProt Protein Name

Methylated-DNA--protein-cysteine methyltransferase

UniProt Synonym Protein Names

6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Protein Family

Probable methylated-DNA--protein-cysteine methyltransferase

UniProt Gene Name

Mgmt

UniProt Synonym Gene Names

MGMT

UniProt Entry Name

MGMT_RAT

NCBI Protein Information

methylated-DNA--protein-cysteine methyltransferase; O6-alkylguanine-DNA alkyltransferase; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA-alkyltransferase; 0-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase

NCBI Summary for Mgmt

enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]

UniProt Comments for Mgmt

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Nuclear receptor co-regulator; Apoptosis; DNA repair, damage; Methyltransferase; EC 2.1.1.63

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding

Biological Process: response to drug; methylation; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA repair; regulation of caspase activity; response to organic cyclic substance; response to DNA damage stimulus

Research Articles on Mgmt

1. Folate deficiency resulted in significant increases in levels of the DNA repair proteins OGG-1 and MGMT in the liver

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Mgmt active protein

Reference Product	PubMed Publications
Mlh1 active protein	>197 publications with Mgmt and Mlh1
Tp53 active protein	>93 publications with Mgmt and Tp53
Pten active protein	>72 publications with Mgmt and Pten
Apex1 active protein	>12 publications with Mgmt and Apex1
Nthl1 active protein	>1 publications with Mgmt and Nthl1

Pathways associated with Mgmt active protein

Products by Pathway	Pathway Diagram
DNA Damage Reversal Pathway antibodies	DNA Damage Reversal Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram

Diseases associated with Mgmt active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1732 publications with Mgmt and Neoplasms
Glioma Antibodies	>776 publications with Mgmt and Glioma
Nervous System Diseases Antibodies	>681 publications with Mgmt and Nervous System Diseases
Brain Neoplasms Antibodies	>664 publications with Mgmt and Brain Neoplasms
Glioblastoma Antibodies	>553 publications with Mgmt and Glioblastoma
Carcinoma Antibodies	>414 publications with Mgmt and Carcinoma
Recurrence Antibodies	>181 publications with Mgmt and Recurrence
Adenocarcinoma Antibodies	>153 publications with Mgmt and Adenocarcinoma
Neoplasms, Experimental Antibodies	>129 publications with Mgmt and Neoplasms, Experimental
Lung Neoplasms Antibodies	>121 publications with Mgmt and Lung Neoplasms

Organs/Tissues associated with Mgmt active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>712 publications with Mgmt and Brain
Prostate Antibodies	>26 publications with Mgmt and Prostate
Ovary Antibodies	>20 publications with Mgmt and Ovary
Heart Antibodies	>5 publications with Mgmt and Heart
Connective Tissue Antibodies	>1 publications with Mgmt and Connective Tissue