• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MIF Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4159945

Macrophage Migration Inhibitory Factor (MIF) is a proinflammatory cytokine which is involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of… More details

$255 / 0.025 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MIF, Active Protein
Full Product Name
Recombinant Mouse MIF
Product Gene Name
MIF active protein
Product Synonym Names
MIF; EC=5.3.2.1; Delayed early response protein 6; DER6; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; EC=5.3.3.12; Phenylpyruvate tautomerase; Recombinant Mouse Macrophage Migration Inhibitory Factor (MIF)
Host
E Coli
Form/Format
Lyophilized from a 0.2 um filtered solution PBS pH 7.4.
Purity/Purification
>95% as determined by SDS-PAGE
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
15.0 kDa
3D Structure
ModBase 3D Structure for P34884
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Bioactivity
The ED(50) was determined by the dose-dependent proliferation of HTB9 cells and was found to be <0.5ng/mL.
Endotoxin Level
<1.0 EU/ug of recombinant protein as determined by the LAL method.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Testing Data of MIF active protein

Mouse MIF Active Protein – Cat# MBS4159945

Testing Data of MIF active protein

Mouse MIF Active Protein, Testing Data – Cat# MBS4159945

Other Notes

Small volumes of MIF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIF purchase

MBS4159945 is an active protein targeting MIF. Also known as Recombinant Mouse MIF. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.

NCBI GI #
6754696
NCBI GeneID
17319
NCBI Accession #
NP_034928.1
UniProt Primary Accession #
P34884
UniProt Related Accession #
P34884
GenBank Nucleotide #
NP_034928.1
NCBI Official Full Name
macrophage migration inhibitory factor
NCBI Official Synonym Full Names
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
NCBI Official Symbol
Mif
NCBI Official Synonym Symbols
GIF; DER6; Glif
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Synonym Protein Names
Delayed early response protein 6; DER6; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
Protein Family
Macrophage migration inhibitory factor
UniProt Gene Name
Mif
UniProt Synonym Gene Names
MIF; DER6; GIF
UniProt Entry Name
MIF_MOUSE
NCBI Protein Information
macrophage migration inhibitory factor

UniProt Comments for MIF

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Apoptosis; Cell development/differentiation; Cytokine; EC 5.3.2.1; EC 5.3.3.12; Isomerase

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; nucleus; vesicle

Molecular Function: chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein complex binding; receptor binding

Biological Process: brain renin-angiotensin system; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; drinking behavior; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive regulation of acute inflammatory response; positive regulation of axon regeneration; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of glucose import; positive regulation of glycolysis; positive regulation of immune response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of potassium ion transport; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell migration; prostaglandin biosynthetic process; regulation of cell proliferation; spermatogenesis
Show more

Research Articles on MIF

1. Treatments with ISO-1 or compound 31 attenuates BLM-induced inflammation and fibrosis in lung, and prevents PH development in mice, suggesting that MIF is an important factor for idiopathic pulmonary fibrosis-Pulmonary hypertension development

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MIF active protein

 Products by Pathway  Pathway Diagram
 Adipogenesis Genes Pathway antibodies  Adipogenesis Genes Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000