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PGAM1 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206433

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PGAM1, Active Protein
Full Product Name
PGAM1, 1-254aa, Human, His-tag, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
Phosphoglycerate mutase 1; Phosphoglycerate mutase isozyme B; PGAM-B; PGAMA
Sequence
MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
30.9 kDa (274aa), confirmed by MALDI-TOF
OMIM
J04173 mRNA
3D Structure
ModBase 3D Structure for P18669
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Product Categories/Family for PGAM1 active protein

Applications Tested/Suitable for PGAM1 active protein
SDS-PAGE

Product References and Citations for PGAM1 active protein

Sakoda S., et al. (1988) J Biol Chem. 263 (32):16899-905. ; Junien C., et al. (1982) Ann Genet. 25 (1):25-7.

Other Notes

Small volumes of PGAM1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGAM1 purchase

MBS206433 is an active protein targeting PGAM1. Also known as PGAM1, 1-254aa, Human, His-tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PGAM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphoglycerate mutase 1 isoform 1
NCBI Official Synonym Full Names
phosphoglycerate mutase 1
NCBI Official Symbol
PGAM1
NCBI Official Synonym Symbols
PGAMA; PGAM-B; HEL-S-35
UniProt Protein Name
Phosphoglycerate mutase 1
UniProt Synonym Protein Names
BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B
UniProt Gene Name
PGAM1
UniProt Synonym Gene Names
PGAMA; PGAM-B
NCBI Protein Information
phosphoglycerate mutase 1

NCBI Summary for PGAM1

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

UniProt Comments for PGAM1

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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