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Platelet Derived Growth Factor Bb Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650024

Recombinant Mouse PDGF-BB is a homodimeric, non-glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 25kD. PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when… More details

$750 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Platelet Derived Growth Factor BB, Active Protein
Full Product Name
Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Le u-Gly-Ser.
Host
E Coli
Form/Format
Supplied as a lyophilized powder from 10mM sodium citrate pH 3.0. Reconstitute in sterile 100mM acetic acid, 0.1% BSA to a concentration 0.1mg/ml, which can then be diluted to other aqueous solutions.
Purity/Purification
Highly Purified
95% as determined by RP-HPLC and SDS-PAGE.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 100mM acetic acid, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
24.45kD
3D Structure
ModBase 3D Structure for A2NWD3
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using an extinction coefficient of 0.48 for a 0.1% solution
2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a reference standard

Other Notes

Small volumes of Platelet Derived Growth Factor BB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Platelet Derived Growth Factor BB purchase

MBS650024 is an active protein targeting Platelet Derived Growth Factor BB. Also known as Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Platelet Derived Growth Factor BB. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
NCBI Official Full Name
platelet-derived growth factor-BB
UniProt Protein Name
Platelet-derived growth factor-BB
UniProt Entry Name
A2NWD3_HUMAN

UniProt Comments for Platelet Derived Growth Factor BB

Sequence similarities: Belongs to the PDGF/VEGF growth factor family. RuleBase RU003818

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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