PPIL3 Active Protein

Name: PPIL3 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204620
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204620

PPIL3, also known as peptidyl-prolyl cis-trans isomerase-like 3, is a member of the cyclophilin family that catalyzes the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. It has been proposed to act either as catalysts or as molecular… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPIL3, Active Protein

Full Product Name

PPIL3, 1-161aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

PPIL3 active protein

Product Synonym Names

Peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3b; CYPJ

Sequence

MGSSHHHHHH SSGLVPRGSH MSVTLHTDVG DIKIEVFCER TPKTCENFLA LCASNYYNGC IFHRNIKGFM VQTGDPTGTG RGGNSIWGKK FEDEYSEYLK HNVRGVVSMA NNGPNTNGSQ FFITYGKQPH LDMKYTVFGK VIDGLETLDE LEKLPVNEKT YRPLNDVHIK DITIHANPFA Q

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Purity/Purification

> 90% by SDS-PAGE

Concentration

1.0 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

20.3 kDa (181aa) confirmed by MALDI-TOF

OMIM

AF251049 mRNA

3D Structure

ModBase 3D Structure for Q9H2H8

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Assay

1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
2. Add 10ul of recombinant PPIL3 protein with 1ug in assay buffer.
3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25C.

Product Categories/Family for PPIL3 active protein

Heat Shock Proteins

Applications Tested/Suitable for PPIL3 active protein

SDS-PAGE

Product References and Citations for PPIL3 active protein

Zhou Z. et al. (2001) Cytogenet Cell Genet. 92: 231-236; Jurica MS. et al. (2002) RNA. 8:426-439.

Other Notes

Small volumes of PPIL3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIL3 purchase

MBS204620 is an active protein targeting PPIL3. Also known as PPIL3, 1-161aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIL3. It may not necessarily be applicable to this product.

NCBI GI #

19557636

NCBI GeneID

53938

NCBI Accession #

NP_570981

UniProt Primary Accession #

Q9H2H8

UniProt Secondary Accession #

Q86WF9; Q96IA9; Q9BXZ1

UniProt Related Accession #

Q9H2H8

GenBank Nucleotide #

NM_130906.2

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3b

NCBI Official Synonym Full Names

peptidylprolyl isomerase like 3

NCBI Official Symbol

PPIL3

NCBI Official Synonym Symbols

CYPJ

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase-like 3

UniProt Synonym Protein Names

Cyclophilin J; CyPJ; Cyclophilin-like protein PPIL3; Rotamase PPIL3

Protein Family

Peptidyl-prolyl cis-trans isomerase

UniProt Gene Name

PPIL3

UniProt Synonym Gene Names

PPIase; CyPJ

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase-like 3

NCBI Summary for PPIL3

This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2008]

UniProt Comments for PPIL3

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.

Research Articles on PPIL3

1. Cyclophilin J expression was up-regulated in gastric carcinoma compared to normal gastric tissues.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PPIL3 active protein

Disease Name	Pubmed Publications
Anemia Antibodies	>1 publications with PPIL3 and Anemia
Carcinoma Antibodies	>1 publications with PPIL3 and Carcinoma
Inflammation Antibodies	>1 publications with PPIL3 and Inflammation

Organs/Tissues associated with PPIL3 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with PPIL3 and Brain
Embryonic Tissue Antibodies	>1 publications with PPIL3 and Embryonic Tissue
Blood Antibodies	>1 publications with PPIL3 and Blood
Muscle Antibodies	>1 publications with PPIL3 and Muscle