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SCF Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9141738

Recombinant Mouse SCF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys26-Ala189) of mouse SCF (Accession #NP_038626.1.) fused with a 6xHis tag at the C-terminus. More details

$220 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SCF, Active Protein
Full Product Name
Recombinant Mouse SCF Protein
Product Gene Name
SCF active protein
Product Synonym Gene Name
KITLG
Product Synonym Names
KITLG; FPH2; KL-1; Kitl; MGF; SCF; SF; SHEP7; KL
Sequence
KEICGNPVTD NVKDITKLVA NLPNDYMITL NYVAGMDVLP SHCWLRDMVI QLSLSLTTLL DKFSNISEGL SNYSIIDKLG KIVDDLVLCM EENAPKNIKE SPKRPETRSF TPEEFFSIFN RSIDAFKDFM VASDTSDCVL SSTLGPEKDS RVSVTKPFML PPVA
Host
HEK293
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Purity/Purification
>95% by SDS-PAGE.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
3D Structure
ModBase 3D Structure for P20826
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Endotoxin
< 0.1 EU/ug of the protein by LAL method.
Biological Activity
Measured by its binding ability in a functional ELISA.Immobilized recombinant Mouse SCF at 2 ug/mL (100 uL/well) can bind recombinant human CD117 with a linear range of 1.5-10ng/mL.
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Tag
6xHis tag at the C-terminus

Product Categories/Family for SCF active protein

Cytokines & Cytokine Receptors
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-Page of SCF active protein

Recombinant Mouse SCF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18-38kDa.
Mouse SCF Active Protein – Cat# MBS9141738

Other Notes

Small volumes of SCF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCF purchase

MBS9141738 is an active protein targeting SCF. Also known as Recombinant Mouse SCF Protein. Host: HEK293.

Additional Details

NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.

NCBI GI #
7305267
NCBI GeneID
17311
NCBI Accession #
NP_038626.1
UniProt Primary Accession #
P20826
UniProt Related Accession #
P20826
GenBank Nucleotide #
NP_038626.1
NCBI Official Full Name
kit ligand isoform 1
NCBI Official Synonym Full Names
kit ligand
NCBI Official Symbol
Kitl
NCBI Official Synonym Symbols
Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
UniProt Protein Name
Kit ligand
UniProt Synonym Protein Names
Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligandSoluble KIT ligand; sKITLG
Protein Family
SCF-associated factor
UniProt Gene Name
Kitlg
UniProt Synonym Gene Names
Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG
UniProt Entry Name
SCF_MOUSE
NCBI Protein Information
kit ligand

UniProt Comments for SCF

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; positive regulation of leukocyte migration; germ cell programmed cell death; germ cell development; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; ovarian follicle development; negative regulation of mast cell apoptosis; positive regulation of melanocyte differentiation; positive regulation of cell proliferation; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication; negative regulation of apoptosis
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Research Articles on SCF

1. data point to transcription factor NRF2 as a major mediator of oxidized palmitoyl-arachidonoyl-phosphatidylcholine -induced upregulation of SCF. This mechanism may represent one of the facets of pleiotropic action of NRF2 in vascular wall.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SCF active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram

Diseases associated with SCF active protein

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >10 publications with SCF and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >6 publications with SCF and Neoplasms, Experimental
 Inflammation Antibodies  >5 publications with SCF and Inflammation
 Weight Loss Antibodies  >3 publications with SCF and Weight Loss
 Fibrosis Antibodies  >3 publications with SCF and Fibrosis
 Metaplasia Antibodies  >3 publications with SCF and Metaplasia
 Infertility, Male Antibodies  >3 publications with SCF and Infertility, Male
 Lung Diseases Antibodies  >3 publications with SCF and Lung Diseases
 Necrosis Antibodies  >3 publications with SCF and Necrosis
 Liver Diseases Antibodies  >2 publications with SCF and Liver Diseases

Organs/Tissues associated with SCF active protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >31 publications with SCF and Bone
 Ovary Antibodies  >26 publications with SCF and Ovary
 Skin Antibodies  >21 publications with SCF and Skin
 Bone Marrow Antibodies  >20 publications with SCF and Bone Marrow
 Testis Antibodies  >18 publications with SCF and Testis
 Embryonic Tissue Antibodies  >9 publications with SCF and Embryonic Tissue
 Liver Antibodies  >8 publications with SCF and Liver
 Heart Antibodies  >6 publications with SCF and Heart
 Muscle Antibodies  >6 publications with SCF and Muscle
 Lung Antibodies  >5 publications with SCF and Lung
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