Sialoprotein II, Bone (IBSP) Active Protein

Name: IBSP Active Protein; Sialoprotein II, Bone; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS636831
Price: 1260 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636831

IBSP (integrinbinding sialoprotein; also BSP or bone sialoprotein II) is a 5575kD, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is principally associated with the early… More details

$1260 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Sialoprotein II, Bone (IBSP), Active Protein

Full Product Name

Sialoprotein II, Bone, Recombinant, Mouse (Bone Sialoprotein 2, Bone Sialoprotein II, BSP II, Cell-binding Sialoprotein, Integrin-binding Sialoprotein, IBSP, BNSP)

Product Gene Name

IBSP active protein

Form/Format

Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.

Purity/Purification

Highly Purified
~90% (SDS-PAGE, reducing conditions)

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

~35kD

Chromosome Location

Chromosome: 4; NC_000004.11 (88720702..88733601). Location: 4q21.1

OMIM

147563

3D Structure

ModBase 3D Structure for P21815

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast adenocarcinoma cells. When 5x10e4 cells/well are added to recombinant mouse IBSP coated plates (3ug/ml with 100ul/well), approximately 60-80% will adhere after 30 minutes at 37 degree C.

Endotoxin Level

1EU/1ug (LAL)

Product Categories/Family for IBSP active protein

Molecular Biology; MB-Extracellular Matrix Proteins

Application Notes for IBSP active protein

SDS-PAGE: 70-90kD, reducing conditions

Other Notes

Small volumes of IBSP active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IBSP purchase

MBS636831 is an active protein targeting IBSP. Also known as Sialoprotein II, Bone, Recombinant, Mouse (Bone Sialoprotein 2, Bone Sialoprotein II, BSP II, Cell-binding Sialoprotein, Integrin-binding Sialoprotein, IBSP, BNSP).

Additional Details

NCBI/Uniprot data below describe general gene information for IBSP. It may not necessarily be applicable to this product.

NCBI GI #

338084

NCBI GeneID

3381

NCBI Accession #

AAC95490.1

UniProt Primary Accession #

P21815

UniProt Related Accession #

P21815

NCBI Official Full Name

sialoprotein

NCBI Official Synonym Full Names

integrin-binding sialoprotein

NCBI Official Symbol

IBSP

NCBI Official Synonym Symbols

BSP; BNSP; SP-II; BSP-II

UniProt Protein Name

Bone sialoprotein 2

UniProt Synonym Protein Names

Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein

Protein Family

Bone sialoprotein

UniProt Gene Name

IBSP

UniProt Synonym Gene Names

BNSP; BSP II

UniProt Entry Name

SIAL_HUMAN

NCBI Protein Information

bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein

NCBI Summary for IBSP

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for IBSP

Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: extracellular space; membrane; extracellular region

Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion

Research Articles on IBSP

1. IBSP mRNA is over expressed in carotid atheroma plaque (3.74 fold, p = 1.41E-09) in an intraindividual comparison.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IBSP active protein

Reference Product	PubMed Publications
SPP1 active protein	>188 publications with IBSP and SPP1
FGF2 active protein	>25 publications with IBSP and FGF2
BGLAP active protein	>11 publications with IBSP and BGLAP

Pathways associated with IBSP active protein

Products by Pathway	Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Interleukin-11 Signaling Pathway antibodies	Interleukin-11 Signaling Pathway Diagram
Osteoblast Signaling Pathway antibodies	Osteoblast Signaling Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram

Diseases associated with IBSP active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>94 publications with IBSP and Neoplasms
Breast Neoplasms Antibodies	>42 publications with IBSP and Breast Neoplasms
Neoplasm Metastasis Antibodies	>24 publications with IBSP and Neoplasm Metastasis
Cardiovascular Diseases Antibodies	>17 publications with IBSP and Cardiovascular Diseases
Kidney Diseases Antibodies	>13 publications with IBSP and Kidney Diseases
Neoplasms, Experimental Antibodies	>12 publications with IBSP and Neoplasms, Experimental
Prostatic Neoplasms Antibodies	>11 publications with IBSP and Prostatic Neoplasms
Lung Diseases Antibodies	>9 publications with IBSP and Lung Diseases
Inflammation Antibodies	>8 publications with IBSP and Inflammation
Mammary Neoplasms, Experimental Antibodies	>6 publications with IBSP and Mammary Neoplasms, Experimental

Organs/Tissues associated with IBSP active protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>643 publications with IBSP and Bone
Connective Tissue Antibodies	>185 publications with IBSP and Connective Tissue
Embryonic Tissue Antibodies	>38 publications with IBSP and Embryonic Tissue
Brain Antibodies	>6 publications with IBSP and Brain