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Stem Cell Factor (KITLG) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS554207

MBS554207 is an active protein targeting Kitlg. Also known as Rat Stem Cell Factor Recombinant. Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.). More details

$240 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Stem Cell Factor (Kitlg), Active Protein
Full Product Name
Rat Stem Cell Factor Recombinant
Product Gene Name
Kitlg active protein
Sequence
MKKTQTWIIT CIYLQLLLFN PLVKTQEICR NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA A SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
Host
E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Form/Format
Recombinant Rat Stemcell factor was lyophilized from a 0.2 um filteredNaHCO3 solution.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
27,681 Da
3D Structure
ModBase 3D Structure for P21581
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
Optimized DNA sequence encoding Rat SCF mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of theproliferation of human TF-1 cells is <=.0 ng/ml, corresponding to a specific activity of >= x units/mg
Molecular Function
Growth-factor
Biological Process
Cell-adhesion
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Product Categories/Family for Kitlg active protein

PDGF family; PDGF family; Recombinant Rat Cytokines

Testing Data of Kitlg active protein

Pathological examination of livers and kidneys after SCF+G-CSF treatment during chronic stroke in aged mice.
S+G 200/50: SCF (200 ?g/kg) in combination with G-CSF (50 ?g/kg).
Rat KITLG Active Protein – Cat# MBS554207

Testing Data of Kitlg active protein

Effect of stem cell factor (SCF) on cardiac stem cell (CSC) migration in vitro.
Representative images of migrated CSCs treated with varying concentrations of SCF by Transwell-based migration assays.
Rat KITLG Active Protein, Testing Data – Cat# MBS554207

Other Notes

Small volumes of Kitlg active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Kitlg. It may not necessarily be applicable to this product.

NCBI GI #
33667068
NCBI GeneID
60427
NCBI Accession #
NP_068615.1
UniProt Primary Accession #
P21581
UniProt Secondary Accession #
Q9QWZ4; Q9Z2E7
UniProt Related Accession #
P21581
GenBank Nucleotide #
NM_021843.4
NCBI Official Full Name
kit ligand isoform 1
NCBI Official Synonym Full Names
KIT ligand
NCBI Official Symbol
Kitlg
NCBI Official Synonym Symbols
Mgf; SCF; Kitl
UniProt Protein Name
Kit ligand
UniProt Synonym Protein Names
Hematopoietic growth factor KL; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligandSoluble KIT ligand; sKITLG
Protein Family
Kit ligand
UniProt Gene Name
Kitlg
UniProt Synonym Gene Names
Kitl; Mgf; MGF; SCF; sKITLG
UniProt Entry Name
SCF_RAT
NCBI Protein Information
kit ligand; C-kit ligand; hematopoietic growth factor KL; mast cell growth factor; steel factor/kit ligand; stem cell factor KL-1

NCBI Summary for Kitlg

soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development [RGD, Feb 2006]

UniProt Comments for Kitlg

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; male gonad development; germ cell programmed cell death; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; ovarian follicle development; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication; negative regulation of apoptosis
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Research Articles on Kitlg

1. A 100 nM dose of E2 unbalanced the SCF/c-kit system, with a crucial impact on germ cell survival and thus male fertility.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Kitlg active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram

Diseases associated with Kitlg active protein

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >3652 publications with Kitlg and Necrosis
 Bone Marrow Diseases Antibodies  >3195 publications with Kitlg and Bone Marrow Diseases
 Carcinoma Antibodies  >2517 publications with Kitlg and Carcinoma
 Inflammation Antibodies  >2073 publications with Kitlg and Inflammation
 Neoplasms, Germ Cell and Embryonal Antibodies  >1790 publications with Kitlg and Neoplasms, Germ Cell and Embryonal
 Heart Diseases Antibodies  >1488 publications with Kitlg and Heart Diseases
 Breast Neoplasms Antibodies  >1382 publications with Kitlg and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1305 publications with Kitlg and Cell Transformation, Neoplastic
 Lung Diseases Antibodies  >1113 publications with Kitlg and Lung Diseases
 Myocardial Ischemia Antibodies  >1102 publications with Kitlg and Myocardial Ischemia

Organs/Tissues associated with Kitlg active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5758 publications with Kitlg and Brain
 Embryonic Tissue Antibodies  >5240 publications with Kitlg and Embryonic Tissue
 Muscle Antibodies  >3847 publications with Kitlg and Muscle
 Heart Antibodies  >2586 publications with Kitlg and Heart
 Lung Antibodies  >1915 publications with Kitlg and Lung
 Adipose Tissue Antibodies  >1433 publications with Kitlg and Adipose Tissue
 Eye Antibodies  >1043 publications with Kitlg and Eye
 Placenta Antibodies  >536 publications with Kitlg and Placenta
 Prostate Antibodies  >424 publications with Kitlg and Prostate
 Ovary Antibodies  >420 publications with Kitlg and Ovary
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