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Syk, Active Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637139

MBS637139 is an active protein targeting Syk, active. Also known as Syk, active, Recombinant, Human. More details

$1360 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Syk, active, Active Protein
Full Product Name
Syk, active, Recombinant, Human
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 270mM sucrose, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF.
Purity/Purification
Purified
~60% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
73kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
195U/mg; One unit of Syk activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30ºC with a final ATP concentration of 100uM.
Application Notes for Syk, active active protein
Suitable for use in MS Tryptic Fingerprint and Kinase Assay.
Dilution: MS Tryptic Fingerprint: Confirmed identity as Syk with translated sequence.
Kinase Assay: 20-55ng phosphorylated 0.1mg/ml poly(Glu, Tyr) (4:1).

Testing Data of Syk, active active protein

Sample enzyme activity data.
Active Protein – Cat# MBS637139

Other Notes

Small volumes of Syk, active active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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