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TFF1 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845286

Background: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. Recombinant human TFF1 is a 8.2 kDa polypeptide containing 60 amino acid… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TFF1, Active Protein
Full Product Name
TFF1, human recombinant
Product Gene Name
Product Synonym Names
pS2 protein; HP1.A; Breast Cancer Estrogen-Inducible protein (BCEI); PNR2; Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; Protein pS2
Host
E Coli
Form/Format
Sterile filtered and lyophilized with no additives
Appearance: Lyophilized protein
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
8.2 kDa
OMIM
113710
3D Structure
ModBase 3D Structure for P04155
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Reconstitute in H?O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers
Endotoxin Level
<0.1 ng/mug
Biological Activity
Determined by its ability to chemoattract human MCF-7 cells using a concentration 5-10 mug/ml.
Results
5-10 mug/ml
Handling
Centrifuge the vial prior to opening.
Applications Tested/Suitable for TFF1 active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of TFF1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFF1 purchase

MBS845286 is an active protein targeting TFF1. Also known as TFF1, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TFF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
trefoil factor 1
NCBI Official Synonym Full Names
trefoil factor 1
NCBI Official Symbol
TFF1
NCBI Official Synonym Symbols
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
UniProt Protein Name
Trefoil factor 1
UniProt Synonym Protein Names
Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2
Protein Family
UniProt Gene Name
TFF1
UniProt Synonym Gene Names
BCEI; PS2; hP1.A
UniProt Entry Name
TFF1_HUMAN
NCBI Protein Information
trefoil factor 1

NCBI Summary for TFF1

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
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UniProt Comments for TFF1

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: response to peptide hormone stimulus; negative regulation of cell proliferation; maintenance of gastrointestinal epithelium; carbohydrate metabolic process; digestion; cell differentiation; response to iron ion; response to estradiol stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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