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TIMP-1 Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9139888

Description: Recombinant Human TIMP-1 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Cys24-Ala207) of human TIMP-1 (Accession #P01033) fused with a 6xHis tag at the C-terminus.Background: This protein… More details

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Product Overview

Product Name
TIMP-1, Active Protein
Full Product Name
Recombinant Human TIMP-1 Protein
Product Gene Name
TIMP-1 active protein
Product Synonym Gene Name
TIMP1
Product Synonym Names
CLGI; EPA; EPO; HCI; TIMP; TIMP-1
Sequence
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
Host
Human Cells
Form/Format
Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Purity/Purification
>95% by SDS-PAGE.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
OMIM
305370
3D Structure
ModBase 3D Structure for P01033
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Endotoxin
< 1 EU/ug of the protein by LAL method.
Biological Activity
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The IC50 value is approximately 2.5 nM.
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Tag
6xHis tag at the C-terminus

Product Categories/Family for TIMP-1 active protein

Cytokines & Cytokine Receptors
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-Page of TIMP-1 active protein

Recombinant protein Human TIMP-1 was determined by SDS-PAGE under reducing conditions with Coomassie Blue, showing a band at 28 kDa.
Human TIMP-1 Active Protein – Cat# MBS9139888

Other Notes

Small volumes of TIMP-1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIMP-1 purchase

MBS9139888 is an active protein targeting TIMP-1. Also known as Recombinant Human TIMP-1 Protein. Host: Human Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMP-1. It may not necessarily be applicable to this product.

NCBI GI #
4507509
NCBI GeneID
7076
NCBI Accession #
NP_003245.1
UniProt Primary Accession #
P01033
UniProt Related Accession #
P01033
GenBank Nucleotide #
NP_003245.1
NCBI Official Full Name
metalloproteinase inhibitor 1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
TIMP1
NCBI Official Synonym Symbols
EPA; EPO; HCI; CLGI; TIMP; TIMP-1
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Synonym Protein Names
Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1
Protein Family
Metalloproteinase inhibitor
UniProt Gene Name
TIMP1
UniProt Synonym Gene Names
CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
UniProt Entry Name
TIMP1_HUMAN
NCBI Protein Information
metalloproteinase inhibitor 1

NCBI Summary for TIMP-1

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]
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UniProt Comments for TIMP-1

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; growth factor activity; metal ion binding; cytokine activity

Biological Process: response to peptide hormone stimulus; platelet activation; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis
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Research Articles on TIMP-1

1. With increased levels of MPO, MMP-8 and MMP-8/TIMP-1 ratio.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TIMP-1 active protein

 Reference Product  PubMed Publications
 MMP2 active protein  >193 publications with TIMP-1 and MMP2
 MMP1 active protein  >100 publications with TIMP-1 and MMP1
 MMP3 active protein  >58 publications with TIMP-1 and MMP3
 CD63 active protein  >5 publications with TIMP-1 and CD63

Pathways associated with TIMP-1 active protein

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with TIMP-1 active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >176 publications with TIMP-1 and Neoplasms
 Fibrosis Antibodies  >108 publications with TIMP-1 and Fibrosis
 Cardiovascular Diseases Antibodies  >91 publications with TIMP-1 and Cardiovascular Diseases
 Inflammation Antibodies  >90 publications with TIMP-1 and Inflammation
 Vascular Diseases Antibodies  >65 publications with TIMP-1 and Vascular Diseases
 Necrosis Antibodies  >57 publications with TIMP-1 and Necrosis
 Liver Diseases Antibodies  >54 publications with TIMP-1 and Liver Diseases
 Heart Diseases Antibodies  >46 publications with TIMP-1 and Heart Diseases
 Female Urogenital Diseases Antibodies  >40 publications with TIMP-1 and Female Urogenital Diseases
 Liver Cirrhosis Antibodies  >32 publications with TIMP-1 and Liver Cirrhosis

Organs/Tissues associated with TIMP-1 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >158 publications with TIMP-1 and Blood
 Vascular Antibodies  >84 publications with TIMP-1 and Vascular
 Liver Antibodies  >81 publications with TIMP-1 and Liver
 Lung Antibodies  >70 publications with TIMP-1 and Lung
 Bone Antibodies  >64 publications with TIMP-1 and Bone
 Muscle Antibodies  >63 publications with TIMP-1 and Muscle
 Heart Antibodies  >53 publications with TIMP-1 and Heart
 Connective Tissue Antibodies  >44 publications with TIMP-1 and Connective Tissue
 Kidney Antibodies  >43 publications with TIMP-1 and Kidney
 Skin Antibodies  >42 publications with TIMP-1 and Skin
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