TPI1 Active Protein

Name: TPI1 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS847025
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS847025

TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TPI1, Active Protein

Full Product Name

TPI1; human recombinant

Product Gene Name

TPI1 active protein

Product Synonym Names

triosephosphate isomerase; TPI

Sequence

MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

Host

E Coli
Source: Human

Form/Format

Liquid
In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol; 1mM DTT

Purity/Purification

>=95%

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C for 12 months.
Centrifuge the vial prior to opening.

Molecular Weight

28.8 kDa

OMIM

M10036 mRNA

3D Structure

ModBase 3D Structure for P60174

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.

Results

>3000 units/mg

Unit Definition

One unit is the amount of enzyme which will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.

Applications Tested/Suitable for TPI1 active protein

SDS-PAGE

Other Notes

Small volumes of TPI1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPI1 purchase

MBS847025 is an active protein targeting TPI1. Also known as TPI1; human recombinant. Host: E Coli
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.

NCBI GI #

4507645

NCBI GeneID

7167

NCBI Accession #

NP_000356.1

UniProt Primary Accession #

P60174

UniProt Secondary Accession #

P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5; B7Z5D8; D3DUS9

UniProt Related Accession #

P60174

GenBank Nucleotide #

NM_000365.5

NCBI Official Full Name

triosephosphate isomerase isoform 1

NCBI Official Synonym Full Names

triosephosphate isomerase 1

NCBI Official Symbol

TPI1

NCBI Official Synonym Symbols

TIM; TPI; TPID; HEL-S-49

UniProt Protein Name

Triosephosphate isomerase

UniProt Synonym Protein Names

Triose-phosphate isomerase

UniProt Gene Name

TPI1

UniProt Synonym Gene Names

TPI; TIM

NCBI Protein Information

triosephosphate isomerase

NCBI Summary for TPI1

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

UniProt Comments for TPI1

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular space; nucleus

Molecular Function: protein binding; triose-phosphate isomerase activity; ubiquitin protein ligase binding

Biological Process: gluconeogenesis; glyceraldehyde-3-phosphate biosynthetic process; glycerol catabolic process; glycolysis

Disease: Triosephosphate Isomerase Deficiency

Research Articles on TPI1

1. Results revealed that TPI expression might be considered as a novel prognostic factor to evaluate gastric cancer patients' survival

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TPI1 active protein

Reference Product	PubMed Publications
PGK1 active protein	>9 publications with TPI1 and PGK1
ENO1 active protein	>8 publications with TPI1 and ENO1
GAPDH active protein	>7 publications with TPI1 and GAPDH
GPI active protein	>3 publications with TPI1 and GPI
PKM2 active protein	>2 publications with TPI1 and PKM2
PKLR active protein	>1 publications with TPI1 and PKLR

Pathways associated with TPI1 active protein

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Fatty Acid Beta Oxidation Pathway antibodies	Fatty Acid Beta Oxidation Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with TPI1 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>10 publications with TPI1 and Neoplasms
Breast Neoplasms Antibodies	>3 publications with TPI1 and Breast Neoplasms
Adenocarcinoma Antibodies	>3 publications with TPI1 and Adenocarcinoma
Hypertension Antibodies	>2 publications with TPI1 and Hypertension
Prostatic Neoplasms Antibodies	>2 publications with TPI1 and Prostatic Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with TPI1 and Carcinoma, Hepatocellular
Drug Toxicity Antibodies	>1 publications with TPI1 and Drug Toxicity
Weight Loss Antibodies	>1 publications with TPI1 and Weight Loss
Abnormalities, Drug-Induced Antibodies	>1 publications with TPI1 and Abnormalities, Drug-Induced
Liver Diseases Antibodies	>1 publications with TPI1 and Liver Diseases

Organs/Tissues associated with TPI1 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with TPI1 and Blood
Brain Antibodies	>4 publications with TPI1 and Brain
Muscle Antibodies	>3 publications with TPI1 and Muscle
Prostate Antibodies	>2 publications with TPI1 and Prostate
Kidney Antibodies	>2 publications with TPI1 and Kidney
Eye Antibodies	>2 publications with TPI1 and Eye
Heart Antibodies	>1 publications with TPI1 and Heart
Liver Antibodies	>1 publications with TPI1 and Liver
Testis Antibodies	>1 publications with TPI1 and Testis
Esophagus Antibodies	>1 publications with TPI1 and Esophagus