TRAIL Active Protein

Name: TRAIL Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844791
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844791

Background: TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TRAIL, Active Protein

Full Product Name

TRAIL, murine recombinant

Product Gene Name

TRAIL active protein

Product Synonym Names

TNF-related apoptosis-inducing Ligand; TNFSF10; Apo2 Ligand; TL2

Sequence

MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN

Host

E Coli

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

20.0 kDa

3D Structure

ModBase 3D Structure for P50592

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Assay #1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml. Assay #2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0 ng/ml.

Biological Activity

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for TRAIL active protein

Applications Tested/Suitable for TRAIL active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of TRAIL active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRAIL purchase

MBS844791 is an active protein targeting TRAIL. Also known as TRAIL, murine recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TRAIL. It may not necessarily be applicable to this product.

NCBI GI #

6678431

NCBI GeneID

22035

NCBI Accession #

NP_033451.1

UniProt Primary Accession #

P50592

UniProt Related Accession #

P50592

GenBank Nucleotide #

NM_009425.2

NCBI Official Full Name

tumor necrosis factor ligand superfamily member 10

NCBI Official Synonym Full Names

tumor necrosis factor (ligand) superfamily, member 10

NCBI Official Symbol

Tnfsf10

NCBI Official Synonym Symbols

TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik

UniProt Protein Name

Tumor necrosis factor ligand superfamily member 10

UniProt Synonym Protein Names

TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253

Protein Family

Tumor necrosis factor ligand superfamily

UniProt Gene Name

Tnfsf10

UniProt Synonym Gene Names

Trail; Protein TRAIL

UniProt Entry Name

TNF10_MOUSE

NCBI Protein Information

tumor necrosis factor ligand superfamily member 10

UniProt Comments for TRAIL

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; immune response

Research Articles on TRAIL

1. These findings indicate that acute fasting enhances TRAIL-mediated liver natural killer cell activity against neoplastic cells through upregulation of heat shock protein 70.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRAIL active protein

Reference Product	PubMed Publications
Tnfrsf10b active protein	>2881 publications with TRAIL and Tnfrsf10b
Casp8 active protein	>1339 publications with TRAIL and Casp8
Fadd active protein	>654 publications with TRAIL and Fadd
Bax active protein	>374 publications with TRAIL and Bax
Cflar active protein	>262 publications with TRAIL and Cflar
Tnfrsf11b active protein	>178 publications with TRAIL and Tnfrsf11b
Bcl2 active protein	>56 publications with TRAIL and Bcl2
Mcl1 active protein	>33 publications with TRAIL and Mcl1

Pathways associated with TRAIL active protein

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Signaling Pathway antibodies	Apoptosis Signaling Pathway Diagram
CASP8 Activity Is Inhibited Pathway antibodies	CASP8 Activity Is Inhibited Pathway Diagram
Caspase Activation Via Extrinsic Apoptotic Signalig Pathway antibodies	Caspase Activation Via Extrinsic Apoptotic Signalig Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Death Receptor Signalling Pathway antibodies	Death Receptor Signalling Pathway Diagram

Diseases associated with TRAIL active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>3960 publications with TRAIL and Necrosis
Leukemia Antibodies	>487 publications with TRAIL and Leukemia
Liver Diseases Antibodies	>356 publications with TRAIL and Liver Diseases
Colorectal Neoplasms Antibodies	>354 publications with TRAIL and Colorectal Neoplasms
Breast Neoplasms Antibodies	>331 publications with TRAIL and Breast Neoplasms
Liver Neoplasms Antibodies	>309 publications with TRAIL and Liver Neoplasms
Lung Neoplasms Antibodies	>303 publications with TRAIL and Lung Neoplasms
Neoplasms, Experimental Antibodies	>302 publications with TRAIL and Neoplasms, Experimental
Inflammation Antibodies	>286 publications with TRAIL and Inflammation
Glioma Antibodies	>254 publications with TRAIL and Glioma

Organs/Tissues associated with TRAIL active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>484 publications with TRAIL and Liver
Lung Antibodies	>409 publications with TRAIL and Lung
Kidney Antibodies	>159 publications with TRAIL and Kidney
Bone Marrow Antibodies	>116 publications with TRAIL and Bone Marrow
Muscle Antibodies	>82 publications with TRAIL and Muscle
Spleen Antibodies	>73 publications with TRAIL and Spleen
Mixed Antibodies	>32 publications with TRAIL and Mixed
Thymus Antibodies	>27 publications with TRAIL and Thymus
Spinal Cord Antibodies	>12 publications with TRAIL and Spinal Cord
Mammary Gland Antibodies	>7 publications with TRAIL and Mammary Gland