WISP3 Active Protein

Name: WISP3 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS553639
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553639

MBS553639 is an active protein targeting WISP3. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

Please Inquire

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

WISP3, Active Protein

Full Product Name

WISP3

Product Gene Name

WISP3 active protein

Sequence

TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L

Host

E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)

Form/Format

Lyophilized; PBS, pH 7.2

Purity/Purification

>95%, as determined by SDS-PAGE and HPLC.

Preparation and Storage

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Molecular Weight

41,402 Da

3D Structure

ModBase 3D Structure for O95389

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Human

Source Note

Optimized DNA sequence encoding Human WISP3 mature chain including a C-terminal 6xHis tag was expressed in ECOLI. Recombinant Human WNT1-inducible-signaling pathway protein 3 encodes amino acid residues 24-354.

Biological Activity

The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.

Endotoxin

Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).

Reconstitution

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Applications Tested/Suitable for WISP3 active protein

SDS-PAGE

Application Notes for WISP3 active protein

Recombinant WISP3 migrates as an approximately 36 kDa protein under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of WISP3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WISP3. It may not necessarily be applicable to this product.

NCBI GI #

38202241

NCBI GeneID

8838

NCBI Accession #

NP_937882.1

UniProt Primary Accession #

O95389

UniProt Secondary Accession #

Q3KR29; Q5H8W4; Q6UXH6

UniProt Related Accession #

O95389

GenBank Nucleotide #

NM_198239.1

NCBI Official Full Name

WNT1-inducible-signaling pathway protein 3 isoform 3

NCBI Official Synonym Full Names

cellular communication network factor 6

NCBI Official Symbol

CCN6

NCBI Official Synonym Symbols

PPD; LIBC; PPAC; WISP3; WISP-3

UniProt Protein Name

WNT1-inducible-signaling pathway protein 3

UniProt Synonym Protein Names

CCN family member 6

Protein Family

WNT1-inducible-signaling pathway protein

UniProt Gene Name

WISP3

UniProt Synonym Gene Names

CCN6; WISP-3

NCBI Protein Information

WNT1-inducible-signaling pathway protein 3

NCBI Summary for WISP3

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for WISP3

Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.

Research Articles on WISP3

1. WISP-3/CCN6 has a role in inhibiting apoptosis by regulating caspase pathway after hyperoxia in lung epithelial cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with WISP3 active protein

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>19 publications with WISP3 and Breast Neoplasms
Arthritis Antibodies	>15 publications with WISP3 and Arthritis
Inflammation Antibodies	>10 publications with WISP3 and Inflammation
Arthropathy, progressive pseudorheumatoid, of childhood Antibodies	>9 publications with WISP3 and Arthropathy, progressive pseudorheumatoid, of childhood
Respiratory Tract Diseases Antibodies	>3 publications with WISP3 and Respiratory Tract Diseases
Congenital Abnormalities Antibodies	>1 publications with WISP3 and Congenital Abnormalities
Diabetes Mellitus Antibodies	>1 publications with WISP3 and Diabetes Mellitus

Organs/Tissues associated with WISP3 active protein

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>17 publications with WISP3 and Connective Tissue