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Bromodeoxyuridine (BrdU) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381463

MBS4381463 is an antibody targeting BrdU. Also known as Bromodeoxyuridine (BrdU) (Proliferation Marker). Reactive in All. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Bromodeoxyuridine (BrdU), Antibody
Full Product Name
Bromodeoxyuridine (BrdU) (Proliferation Marker)
Product Gene Name
anti-BrdU antibody
Product Synonym Names
Bromodeoxyuridine; BUdr
Isotype
IgG1, kappa
Clone Number
rBRD.3
Host
Mouse
Species Reactivity
All
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
Depends on the target
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Immunogen
Bromodeoxyuridine (BrdU) conjugated to BSA
Cellular Localization
Nuclear
Antibody Type
Recombinant Mouse Monoclonal Antibody
Applications Tested/Suitable for anti-BrdU antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-BrdU antibody
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37 degree C]

SDS-Page of anti-BrdU antibody

SDS-PAGE Analysis Puriifed BrdU Recombinant Mouse Monoclonal Antibody (rBRD.3). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-BrdU Antibody – Cat# MBS4381463

Product References and Citations for anti-BrdU antibody

1. Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6. 2. Williams LS et. al. Cytometry, 1990, 11(4):490-7

Other Notes

Small volumes of anti-BrdU antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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