Cortisol Assay Kit

Principle of the Assay: This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of Cortisol in biological samples such as urine, saliva, and pl'csma. Briefly, cortisol in the samples or standards competes with cortisol conjugated to horseradish peroxidase (HRP) for binding to a polycJonal antibody specific for cortisol coated on the microplate. The HRP activity results in brilIiant blue color development when the substrate is added, with the intensity of the color proportional to the amount of cortisol-HRP bound and inversely proportional to the amount of unconjugated cortisol in the samples or standards.

Background: is produced throughout the day; with very high levels in the early morning it quickly decreases to a low level in the early afternoon. After that, it continues to slowly decrease into the night quickly spiking before morning. Cortisol levels may also spike immediately after waking up, during periods of low blood glucose levels, and in response to stress. Cortisol is also known as Hydrocortisone when it is used medicinally, usually as a supplement or for its anti- inflammatory properties. Knowing plasma Cortisol levels is useful in identifying a serious lack of cortisol usually found in Addison's disease, or a serious overproduction of cortisol as in Cushing's syndrome. Cortisol is also frequently used to evaluate the stress levels of a subject.

Small volumes of Cortisol assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS480401 is an assay kit targeting Cortisol. Also known as Cortisol EIA Kit.