Fibrinogen (FGG) Assay Kit

Name: FGG Assay Kit; Fibrinogen; MyBioSource
Brand: MyBioSource
SKU: MBS480412
Price: 760 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS480412

Introduction: Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade 1. Hepatic expression of fibrinogen increases two to four hundred fold during the… More details

$760 / 1 Kit +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Fibrinogen (FGG), Assay Kit

Full Product Name

Rat Fibrinogen ELISA assay

Product Gene Name

FGG assay kit

Product Synonym Names

Rat Fibrinogen Antigen Assay

Preparation and Storage

1. Store the kit and all of its components at 4 degree C before use.
2. If not using the entire plate at once, prepare only the appropriate amount of Primary Antibody and Standard. The remaining stock solutions should be frozen and stored at At -70 degree C. Do not freeze/thaw more than once. All other components should remain refrigerated.
3. Store unused portions of the microplate in a pouch with a desiccant at 4 degree C.

Molecular Weight

50,244 Da

3D Structure

ModBase 3D Structure for P12799

Procedural Notes

1. This assay should be run at room temperature.
2. Use aseptic technique when opening and dispensing reagents.
3. This kit is designed to work properly as provided and instructed. Additions, deletions or substitutions to the procedure or reagents are not recommended, as they may be detrimental to the assay.
4. To minimize error due to handling, wipe the exterior bottom of the microplate wells with a lint-free paper towel.

Reagent Preparation

1. 10x Wash Buffer: Dilute the 50 mL of concentrate to 1x with 450 mL of DI water prior to use.
2. 5x Dilution Buffer: Dilute the 50 mL of concentrate to 1x with 200 mL of DI water prior to use.
3. Primary Antibody: Reconstitute with 1x Dilution Buffer as directed on the vial and vortex gently to mix. Prepare immediately prior to use.
4. Secondary Conjugate: Dilute with 1x Dilution Buffer as directed on the vial and vortex gently to mix. Prepare immediately prior to use.

Standard Preparation

Reconstitute the standard by adding 5.0 mL of dilution buffer directly to the vial and agitate to fully dissolve. This will provide a concentration of 800 ng/mL solution. Prepare the serial dilution as outlined in the table below.
Standard Fibrinogen Concentration (ng/mL) Amount of 1x Dilution Buffer (muL) Transfer Volume (muL) Transfer Source Total Volume (muL)
S9 800 --- 1000 Stock Vial 500
S8 400 500 500 S9 500
S7 200 500 500 S8 500
S6 100 500 500 S7 500
S5 50 500 500 S6 500
S4 25 500 500 S5 500
S3 12.5 500 500 S4 500
S2 6.25 500 500 S3 500
S1 3.125 500 500 S2 500
S0 0 500 ---- --- 500

Sample Collection, Storage and Preparation

Samples giving rat fibrinogen levels above 800 ng/ml should be diluted in 1X Dilution Buffer before use. Normal plasma samples need to be diluted between 1:10,000 and 1:50,000 in 1X Dilution Buffer for the values to be within the linear range of the standard curve.

Product References and Citations for FGG assay kit

1. Kamath, S. and Lip, G.Y.H.; (2003) QJM 96:711-729
2. Kusher, I.; (1982) Ann New York Acad Sci 389:39-48
3. Kannel, W.B., et al.; (1987) J Am Med Assoc 258:1183-1186
4. Handa, K., et al.; (1989) Atherosclerosis 77:209-213
5. Larrson, A., et al.; (1997) Vet Immunol Immunopathol 59:163-169
6. Dorner, H., et al., (1995) Gerontology 41:252-259

Other Notes

Small volumes of FGG assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FGG purchase

MBS480412 is an assay kit targeting FGG. Also known as Rat Fibrinogen ELISA assay.

Additional Details

NCBI/Uniprot data below describe general gene information for FGG. It may not necessarily be applicable to this product.

NCBI GI #

350

NCBI GeneID

280792

NCBI Accession #

CAA33562.1

UniProt Primary Accession #

P12799

UniProt Related Accession #

P12799

NCBI Official Full Name

fibrinogen

NCBI Official Symbol

FGG

UniProt Protein Name

Fibrinogen gamma-B chain

Protein Family

Fibrinogen

UniProt Gene Name

FGG

UniProt Synonym Gene Names

Gamma'

UniProt Entry Name

FIBG_BOVIN

NCBI Protein Information

fibrinogen gamma-B chain; fibrinogen, gamma polypeptide; gamma'

UniProt Comments for FGG

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FGG assay kit

Reference Product	PubMed Publications
FGA assay kit	>86 publications with FGG and FGA
FGB assay kit	>84 publications with FGG and FGB
F13A1 assay kit	>4 publications with FGG and F13A1
ITGB3 assay kit	>2 publications with FGG and ITGB3
ITGA2B assay kit	>1 publications with FGG and ITGA2B
ITGB2 assay kit	>1 publications with FGG and ITGB2

Pathways associated with FGG assay kit

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Platelet Activation Pathway antibodies	Platelet Activation Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram

Diseases associated with FGG assay kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>164 publications with FGG and Cardiovascular Diseases
Brain Diseases Antibodies	>93 publications with FGG and Brain Diseases
Heart Diseases Antibodies	>82 publications with FGG and Heart Diseases
Death Antibodies	>57 publications with FGG and Death
Lung Diseases Antibodies	>45 publications with FGG and Lung Diseases
Inflammation Antibodies	>37 publications with FGG and Inflammation
Thrombosis Antibodies	>36 publications with FGG and Thrombosis
Liver Diseases Antibodies	>33 publications with FGG and Liver Diseases
Kidney Diseases Antibodies	>27 publications with FGG and Kidney Diseases
Hypertension Antibodies	>20 publications with FGG and Hypertension

Organs/Tissues associated with FGG assay kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>74 publications with FGG and Brain
Muscle Antibodies	>58 publications with FGG and Muscle
Liver Antibodies	>54 publications with FGG and Liver
Embryonic Tissue Antibodies	>14 publications with FGG and Embryonic Tissue
Testis Antibodies	>13 publications with FGG and Testis
Reticulum Antibodies	>7 publications with FGG and Reticulum