Hydroxyproline (HYP) Assay Kit

Background: Hydroxyproline (HYP), one of the sub-amino acids and the main components of collagen tissue, is the unique amino acid in collagen. Collagen tissue is mostly distributed in skin, tendon, blood vessel, cartilage and connective tissue of human body. Many diseases can be accompanied by changes in collagen metabolism, resulting in changes in the content of HYP in blood, urine and tissues. The measurement of HYP can be used to judge the catabolism of connective tissue and the degree of fibrosis in the body.

Principle of the Assay: The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558nm.

Small volumes of HYP assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2570523 is an assay kit targeting HYP. Also known as Hydroxyproline (HYP) Colorimetric Assay Kit.