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PHF5A Antibody (C-Term) Blocking Peptide

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9219957

Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER- alpha. Also involved in pre-mRNA splicing. More details

$455 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PHF5A, Blocking Peptide
Full Product Name
PHF5A Antibody (C-term) Blocking peptide
Product Gene Name
Product Synonym Names
PHD finger-like domain-containing protein 5A; PHD finger-like domain protein 5A; Splicing factor 3B-associated 14 kDa protein; SF3b14b; PHF5A
Form/Format
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
Specificity
The synthetic peptide sequence used to generate the antibody was selected from the C-term region of PHF5A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Antibody/Peptide Pairs
PHF5A peptide (MBS9219957) is used for blocking the activity of PHF5A antibody (MBS9207454)
Molecular Weight
12,405 Da
Cellular Location
Nucleus. Nucleus speckle

Other Notes

Small volumes of PHF5A blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHF5A purchase

MBS9219957 is a blocking peptide targeting PHF5A. Also known as PHF5A Antibody (C-term) Blocking peptide.

Additional Details

NCBI/Uniprot data below describe general gene information for PHF5A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
PHD finger-like domain-containing protein 5A
NCBI Official Synonym Full Names
PHD finger protein 5A
NCBI Official Symbol
PHF5A
NCBI Official Synonym Symbols
INI; Rds3; SF3B7; SAP14b; SF3b14b; bK223H9.2
UniProt Protein Name
PHD finger-like domain-containing protein 5A
UniProt Synonym Protein Names
Splicing factor 3B-associated 14 kDa protein; SF3b14b
UniProt Gene Name
PHF5A
UniProt Synonym Gene Names
PHD finger-like domain protein 5A; SF3b14b
UniProt Entry Name
PHF5A_HUMAN
NCBI Protein Information
PHD finger-like domain-containing protein 5A

NCBI Summary for PHF5A

This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008]
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UniProt Comments for PHF5A

PHF5A: Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER- alpha. Also involved in pre-mRNA splicing. Belongs to the PHF5 family.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nuclear speck; nucleoplasm; snRNP U2; U12-dependent spliceosome

Molecular Function: transcription factor activity

Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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