ELISA Wash Buffer

Description: ELISA Wash Buffer, 10X effectively rinses microtiter plates between reagent addition steps of an ELISA to remove signal-altering debris yet preserve assay components.

Background: ELISA Wash Buffer formulation is used to rinse microtiter plates during the coating process and between reagent addition steps of an ELISA.

This buffer is an optimal formulation of pH stabilizers, salts, and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound assay components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background noise and increasing the specific signal.

ELISA Wash Buffer may be used with antibody-sandwich ELISAs and antigen-down ELISAs, and is compatible with all routinely used conjugate components such as HRP, AP, and avidin, among others. ELISA Wash Buffer contains a non-azide, non-mercury preservative that will not interfere with antibody-antigen binding interactions.

Small volumes of ELISA Wash Buffer buffer vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS258403 is a buffer targeting ELISA Wash Buffer. Also known as ELISA Wash Buffer, 10X.