Complement Fragment 5a (C5A) ELISA Kit for Sheep

Name: Sheep C5A ELISA Kit; Complement Fragment 5a; MyBioSource
Brand: MyBioSource
SKU: MBS018339
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS018339

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of C5A (hereafter termed "analyte") in undiluted original Sheep body fluids, tissue homogenates,… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Complement Fragment 5A (C5A), ELISA Kit

Full Product Name

Sheep Complement Fragment 5A ELISA Kit

Product Gene Name

C5A elisa kit

Species Reactivity

Sheep

Assay Type

Sandwich

Samples

Undiluted original Sheep body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

Detection Range

6.25 ng/ml - 200 ng/ml.

Sensitivity

1.0ng/ml.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

36,080 Da

OMIM

609949

3D Structure

ModBase 3D Structure for Q9P296

Other Notes

Small volumes of C5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C5A purchase

MBS018339 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Fragment 5A (C5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C5A. The ELISA analytical biochemical technique of the MBS018339 kit is based on C5A antibody-C5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for C5A. It may not necessarily be applicable to this product.

NCBI GI #

8923873

NCBI GeneID

27202

NCBI Accession #

NP_060955.1

UniProt Primary Accession #

Q9P296

UniProt Secondary Accession #

B2RA09

UniProt Related Accession #

Q9P296

GenBank Nucleotide #

NM_018485.2

NCBI Official Full Name

C5a anaphylatoxin chemotactic receptor 2

NCBI Official Synonym Full Names

complement component 5a receptor 2

NCBI Official Symbol

C5AR2

NCBI Official Synonym Symbols

C5L2; GPF77; GPR77

UniProt Protein Name

C5a anaphylatoxin chemotactic receptor 2

UniProt Synonym Protein Names

Complement component 5a receptor 2; G-protein coupled receptor 77

UniProt Gene Name

C5AR2

UniProt Synonym Gene Names

C5L2; GPR77

UniProt Entry Name

C5AR2_HUMAN

NCBI Protein Information

C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2

NCBI Summary for C5A

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

UniProt Comments for C5A

C5AR2: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: apical part of cell; basal plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; C5a anaphylatoxin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of tumor necrosis factor production; chemotaxis; inflammatory response; positive regulation of epithelial cell proliferation

Research Articles on C5A

1. A novel polymorphism (901G > a) of C5L2 gene is associated with coronary artery disease in Chinese Han and Uyghur population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with C5A elisa kit

Reference Product	PubMed Publications
C5AR1 elisa kit	>1 publications with C5A and C5AR1

Pathways associated with C5A elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
GPCRs, Other Pathway antibodies	GPCRs, Other Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Organs/Tissues associated with C5A elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with C5A and Brain