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CD81 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800939

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and… More details

$315 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD81, Antibody
Full Product Name
Anti-Hu CD81 Purified Low Endotoxin
Product Gene Name
Product Synonym Names
CD81 molecule; S5.7; CVID6; TAPA1; TSPAN28
Isotype
IgG1
Clone Number
M38
Host
Mouse
Species Reactivity
Rabbit, Cat, Human
Form/Format
Purified Low Endotoxin
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Preparation and Storage
Store at 2°C to 8°C. Do not freeze. Do not use after expiration date stamped on the label.
Matching Pairs
Unconjugated Antibody: CD81 Clone #M38 (MBS1800938)
Unconjugated Low Endotoxin Antibody: CD81 Clone #M38 (MBS1800939)
OMIM
M33680 mRNA
3D Structure
ModBase 3D Structure for P60033
Immunogen
MOLT-4 (human T-ALL cell line)
Antigen
CD81
Applications Tested/Suitable for anti-CD81 antibody
Flow Cytometry (FC)(QC Tested), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-p) , Immunocytochemistry (ICC), Functional
Application Notes for anti-CD81 antibody
Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: recommended dilution: 1 ug/ml.
Western blotting: recommended dilution: 1-2 ug/ml; positive control: Jurkat cells, non-reducing conditions.

Western Blot (WB) of anti-CD81 antibody

Western blotting analysis of human CD81 using mouse monoclonal antibody M38 on lysate of Jurkat cell line under non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse anti-human CD81 monoclonal antibody M38 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD81 at approximately 25 kDa.
Mouse Anti-CD81 Antibody – Cat# MBS1800939

Flow Cytometry (FC/FACS) of anti-CD81 antibody

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.
Mouse Anti-CD81 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800939

Flow Cytometry (FC/FACS) of anti-CD81 antibody

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.
Mouse Anti-CD81 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800939

Other Notes

Small volumes of anti-CD81 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD81 purchase

MBS1800939 is an antibody targeting CD81. Also known as Anti-Hu CD81 Purified Low Endotoxin. Reactive in Rabbit, Cat, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD81. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD81 antigen
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
UniProt Protein Name
CD81 antigen
UniProt Synonym Protein Names
26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD_antigen: CD81
Protein Family
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28
NCBI Protein Information
CD81 antigen

NCBI Summary for CD81

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
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UniProt Comments for CD81

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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