Human BUB1 cDNA Clone Expression Plasmid – Cat# MBS1267055

BUB1 cDNA Clone – Budding Uninhibited by Benzimidazoles 1 Homolog (Yeast) (Human)

Name: BUB1 cDNA Clone; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1267055
Price: 1410 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1267055

MBS1267055 is a cDNA clone targeting BUB1. More details

$1410 / 0.01 mg Plasmid + 0.2 mL Glycerol-Stock +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BUB1, cDNA Clone

Full Product Name

BUB1 cDNA Clone

Product Gene Name

BUB1 cdna clone

Sequence

atggacaccc cggaaaatgt ccttcagatg cttgaagccc acatgcagag ctacaagggc aatgaccctc ttggtgaatg ggaaagatac atacagtggg tagaagagaa ttttcctgag aataaagaat acttgataac tttactagaa catttaatga aggaattttt agataagaag aaataccaca atgacccaag attcatcagt tattgtttaa aatttgctga gtacaacagt gacctccatc aattttttga gtttctgtac aaccatggga ttggaaccct gtcatcccct ctgtacattg cctgggcggg gcatctggaa gcccaaggag agctgcagca tgccagtgct gtccttcaga gaggaattca aaaccaggct gaacccagag agttcctgca acaacaatac aggttatttc agacacgcct cactgaaacc catttgccag ctcaagctag aacctcagaa cctctgcata atgttcaggt tttaaatcaa atgataacat caaaatcaaa tccaggaaat aacatggcct gcatttctaa gaatcagggt tcagagcttt ctggagtgat atcttcagct tgtgataaag agtcaaatat ggaacgaaga gtgatcacga tttctaaatc agaatattct gtgcactcat ctttggcatc caaagttgat gttgagcagg ttgttatgta ttgcaaggag aagcttattc gtggggaatc agaattttcc tttgaagaat tgagagccca gaaatacaat caacggagaa agcatgagca atgggtaaat gaagacagac attatatgaa aaggaaagaa gcaaatgctt ttgaagaaca gctattaaaa cagaaaatgg atgaacttca taagaagttg catcaggtgg tggagacatc ccatgaggat ctgcccgctt cccaggaaag gtccgaggtt aatccagcac gtatggggcc aagtgtaggc tcccagcagg aactgagagc gccatgtctt ccagtaacct atcagcagac accagtgaac atggaaaaga acccaagaga ggcacctcct gttgttcctc ctttggcaaa tgctatttct gcagctttgg tgtccccagc caccagccag agcattgctc ctcctgttcc tttgaaagcc cagacagtaa cagactccat gtttgcagtg gccagcaaag atgctggatg tgtgaataag agtactcatg aattcaagcc acagagtgga gcagagatca aagaagggtg tgaaacacat aaggttgcca acacaagttc ttttcacaca actccaaaca catcactggg aatggttcag gcaacgccat ccaaagtgca gccatcaccc accgtgcaca caaaagaagc attaggtttc atcatgaata tgtttcaggc tcctacactt cctgatattt ctgatgacaa agatgaatgg caatctctag atcaaaatga agatgcattt gaagcccagt ttcaaaaaaa tgtaaggtca tctggggctt ggggagtcaa taagatcatc tcttctttgt catctgcttt tcatgtgttt gaagatggaa acaaagaaaa ttatggatta ccacagccta aaaataaacc cacaggagcc aggacctttg gagaacgctc tgtcagcaga cttccttcaa aaccaaagga ggaagtgcct catgctgaag agtttttgga tgactcaact gtatggggta ttcgctgcaa caaaaccctg gcacccagtc ctaagagccc aggagacttc acatctgctg cacaacttgc gtctacacca ttccacaagc ttccagtgga gtcagtgcac attttagaag ataaagaaaa tgtggtagca aaacagtgta cccaggcgac tttggattct tgtgaggaaa acatggtggt gccttcaagg gatggaaaat tcagtccaat tcaagagaaa agcccaaaac aggccttgtc gtctcacatg tattcagcat ccttacttcg tctgagccag cctgctgcag gtggggtact tacctgtgag gcagagttgg gcgttgaggc ttgcagactc acagacactg acgctgccat tgcagaagat ccaccagatg ctattgctgg gctccaagca gaatggatgc agatgagttc acttgggact gttgatgctc caaacttcat tgttgggaac ccatgggatg ataagctgat tttcaaactt ttatctgggc tttctaaacc agtgagttcc tatccaaata cttttgaatg gcaatgtaaa cttccagcca tcaagcccaa gactgaattt caattgggtt ctaagctggt ctatgtccat caccttcttg gagaaggagc ctttgcccag gtgtacgaag ctacccaggg agatctgaat gatgctaaaa ataaacagaa atttgtttta aaggtccaaa agcctgccaa cccctgggaa ttctacattg ggacccagtt gatggaaaga ctaaagccat ctatgcagca catgtttatg aagttctatt ctgcccactt attccagaat ggcagtgtat tagtaggaga gctctacagc tatggaacat tattaaatgc cattaacctc tataaaaata cccctgaaaa agtgatgcct caaggtcttg tcatctcttt tgctatgaga atgctttaca tgattgagca agtgcatgac tgtgaaatca ttcatggaga cattaaacca gacaatttca tacttggaaa cggatttttg gaacaggatg atgaagatga tttatctgct ggcttggcac tgattgacct gggtcagagt atagatatga aactttttcc aaaaggaact atattcacag caaagtgtga aacatctggt tttcagtgtg ttgagatgct cagcaacaaa ccatggaact accagatcga ttactttggg gttgctgcaa cagtatattg catgctcttt ggcacttaca tgaaagtgaa aaatgaagga ggagagtgta agcctgaagg tctttttaga aggcttcctc atttggatat gtggaatgaa ttttttcatg ttatgttgaa tattccagat tgtcatcatc ttccatcttt ggatttgtta aggcaaaagc tgaagaaagt atttcaacaa cactatacta acaagattag ggccctacgt aataggctaa ttgtactgct cttagaatgt aagcgttcac gaaaataa

Molecular Weight

120,002 Da

OMIM

602452

Vector

pUC

Clone Sequence Report

Provided with product shipment

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of BUB1 cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BUB1. It may not necessarily be applicable to this product.

NCBI GI #

20381367

NCBI GeneID

699

NCBI Accession #

BC028201

UniProt Secondary Accession #

O43430; O43643; O60626; Q53QE4; E9PC26; F5GXI5

UniProt Related Accession #

O43683

NCBI Official Full Name

Homo sapiens budding uninhibited by benzimidazoles 1 homolog (yeast), mRNA

NCBI Official Synonym Full Names

BUB1 mitotic checkpoint serine/threonine kinase

NCBI Official Symbol

BUB1

NCBI Official Synonym Symbols

BUB1A; BUB1L; hBUB1

UniProt Protein Name

Mitotic checkpoint serine/threonine-protein kinase BUB1

UniProt Synonym Protein Names

BUB1A

Protein Family

Checkpoint serine/threonine-protein kinase

UniProt Gene Name

BUB1

UniProt Synonym Gene Names

BUB1L; hBUB1

UniProt Entry Name

BUB1_HUMAN

NCBI Protein Information

mitotic checkpoint serine/threonine-protein kinase BUB1

NCBI Summary for BUB1

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

UniProt Comments for BUB1

BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.

Protein type: Kinase, protein; Protein kinase, Other; Cell cycle regulation; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: cytoplasm; cytosol; kinetochore; membrane; nucleoplasm

Molecular Function: protein binding; protein kinase activity

Biological Process: mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; sister chromatid cohesion

Research Articles on BUB1

1. Bub1-Plk1-mediated phosphorylation of Cdc20 constitutes an anaphase-promoting complex or cyclosome-inhibitory mechanism that is parallel, but not redundant, to mitotic checkpoint complex formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with BUB1 cdna clone

Products by Pathway	Pathway Diagram
Aurora B Signaling Pathway antibodies	Aurora B Signaling Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Mitotic Prometaphase Pathway antibodies	Mitotic Prometaphase Pathway Diagram

Diseases associated with BUB1 cdna clone

Disease Name	Pubmed Publications
Neoplasms Antibodies	>182 publications with BUB1 and Neoplasms
Carcinoma Antibodies	>61 publications with BUB1 and Carcinoma
Breast Neoplasms Antibodies	>27 publications with BUB1 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>22 publications with BUB1 and Cell Transformation, Neoplastic
Lung Neoplasms Antibodies	>17 publications with BUB1 and Lung Neoplasms
Colonic Neoplasms Antibodies	>14 publications with BUB1 and Colonic Neoplasms
Ovarian Neoplasms Antibodies	>13 publications with BUB1 and Ovarian Neoplasms
Carcinoma, Squamous Cell Antibodies	>11 publications with BUB1 and Carcinoma, Squamous Cell
Neoplasms, Experimental Antibodies	>10 publications with BUB1 and Neoplasms, Experimental
Drug Toxicity Antibodies	>9 publications with BUB1 and Drug Toxicity

Organs/Tissues associated with BUB1 cdna clone

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>22 publications with BUB1 and Lung
Blood Antibodies	>16 publications with BUB1 and Blood
Brain Antibodies	>13 publications with BUB1 and Brain
Stomach Antibodies	>11 publications with BUB1 and Stomach
Lymph Antibodies	>9 publications with BUB1 and Lymph
Liver Antibodies	>9 publications with BUB1 and Liver
Lymph Node Antibodies	>9 publications with BUB1 and Lymph Node
Bone Antibodies	>8 publications with BUB1 and Bone
Prostate Antibodies	>8 publications with BUB1 and Prostate
Muscle Antibodies	>8 publications with BUB1 and Muscle