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LIG4 cDNA Clone – Ligase IV, DNA, ATP-Dependent (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1267837

MBS1267837 is a cDNA clone targeting LIG4. More details

$630 / 0.01 mg Plasmid + 0.2 mL Glycerol-Stock +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LIG4, cDNA Clone
Full Product Name
LIG4 cDNA Clone
Product Gene Name
LIG4 cdna clone
Sequence
atggctgcct cacaaacttc acaaactgtt gcatctcacg ttccttttgc agatttgtgt tcaactttag aacgaataca gaaaagtaaa ggacgtgcag aaaaaatcag acacttcagg gaatttttag attcttggag aaaatttcat gatgctcttc ataagaacca caaagatgtc acagactctt tttatccagc aatgagacta attcttcctc agctagaaag agagagaatg gcctatggaa ttaaagaaac tatgcttgct aagctttata ttgagttgct taatttacct agagatggaa aagatgccct caaactttta aactacagaa cacccactgg aactcatgga gatgctggag actttgcaat gattgcatat tttgtgttga agccaagatg tttacagaaa ggaagtttaa ccatacagca agtaaacgac cttttagact caattgccag caataattct gctaaaagaa aagacctaat aaaaaagagc cttcttcaac ttataactca gagttcagca cttgagcaaa agtggcttat acggatgatc ataaaggatt taaagcttgg tgttagtcag caaactatct tttctgtttt tcataatgat gctgctgagt tgcataatgt cactacagat ctggaaaaag tctgtaggca actgcatgat ccttctgtag gactcagtga tatttctatc actttatttt ctgcatttaa accaatgcta gctgctattg cagatattga gcacattgag aaggatatga aacatcagag tttctacata gaaaccaagc tagatggtga acgtatgcaa atgcacaaag atggagatgt atataaatac ttctctcgaa atggatataa ctacactgat cagtttggtg cttctcctac tgaaggttct cttaccccat tcattcataa tgcattcaaa gcagatatac aaatctgtat tcttgatggt gagatgatgg cctataatcc taatacacaa actttcatgc aaaagggaac taagtttgat attaaaagaa tggtagagga ttctgatctg caaacttgtt attgtgtttt tgatgtattg atggttaata ataaaaagct agggcatgag actctgagaa agaggtatga gattcttagt agtattttta caccaattcc aggtagaata gaaatagtgc agaaaacaca agctcatact aagaatgaag taattgatgc attgaatgaa gcaatagata aaagagaaga gggaattatg gtaaaacaac ctctatccat ctacaagcca gacaaaagag gtgaagggtg gttaaaaatt aaaccagagt atgtcagtgg actaatggat gaattggaca ttttaattgt tggaggatat tggggtaaag gatcacgggg tggaatgatg tctcattttc tgtgtgcagt agcagagaag ccccctcctg gtgagaagcc atctgtgttt catactctct ctcgtgttgg gtctggctgc accatgaaag aactgtatga tctgggtttg aaattggcca agtattggaa gccttttcat agaaaagctc caccaagcag cattttatgt ggaacagaga agccagaagt atacattgaa ccttgtaatt ctgtcattgt tcagattaaa gcagcagaga tcgtacccag tgatatgtat aaaactggct gcaccttgcg ttttccacga attgaaaaga taagagatga caaggagtgg catgagtgca tgaccctgga cgacctagaa caacttaggg ggaaggcatc tggtaagctc gcatctaaac acctttatat aggtggtgat gatgaaccac aagaaaaaaa gcggaaagct gccccaaaga tgaagaaagt tattggaatt attgagcact taaaagcacc taaccttact aacgttaaca aaatttctaa tatatttgaa gatgtagagt tttgtgttat gagtggaaca gatagccagc caaagcctga cctggagaac agaattgcag aatttggtgg ttatatagta caaaatccag gcccagacac gtactgtgta attgcagggt ctgagaacat cagagtgaaa aacataattt tgtcaaataa acatgatgtt gtcaagcctg catggctttt agaatgtttt aagaccaaaa gctttgtacc atggcagcct cgctttatga ttcatatgtg cccatcaacc aaagaacatt ttgcccgtga atatgattgc tatggtgata gttatttcat tgatacagac ttgaaccaac tgaaggaagt attctcagga attaaaaatt ctaacgagca gactcctgaa gaaatggctt ctctgattgc tgatttagaa tatcggtatt cctgggattg ctctcctctc agtatgtttc gacgccacac cgtttatttg gactcgtatg ctgttattaa tgacctgagt accaaaaatg aggggacaag gttagctatt aaagccttgg agcttcggtt tcatggagca aaagtagttt cttgtttagc tgagggagtg tctcatgtaa taattgggga agatcatagt cgtgttgcag attttaaagc ttttagaaga acttttaaga gaaagtttaa aatcctaaaa gaaagttggg taactgattc aatagacaag tgtgaattac aagaagaaaa ccagtatttg atttaa
Molecular Weight
103,971 Da
OMIM
601837
Vector
pENTR223.1 or pUC
Clone Sequence Report
Provided with product shipment
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LIG4 cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LIG4. It may not necessarily be applicable to this product.

NCBI GI #
34190693
NCBI GeneID
3981
NCBI Accession #
BC037491
UniProt Secondary Accession #
Q8IY66; Q8TEU5
UniProt Related Accession #
P49917
NCBI Official Full Name
Homo sapiens ligase IV, DNA, ATP-dependent, mRNA
NCBI Official Synonym Full Names
DNA ligase 4
NCBI Official Symbol
LIG4
NCBI Official Synonym Symbols
LIG4S
UniProt Protein Name
DNA ligase 4
UniProt Synonym Protein Names
DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Protein Family
DNA ligase
UniProt Gene Name
LIG4
UniProt Entry Name
DNLI4_HUMAN
NCBI Protein Information
DNA ligase 4

NCBI Summary for LIG4

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for LIG4

LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome.

Protein type: EC 6.5.1.1; Ligase

Chromosomal Location of Human Ortholog: 13q33-q34

Cellular Component: condensed chromosome; cytoplasm; DNA ligase IV complex; DNA-dependent protein kinase complex; focal adhesion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; plasma membrane

Molecular Function: DNA binding; DNA ligase (ATP) activity; DNA ligase activity; ligase activity; protein binding; protein C-terminus binding

Biological Process: cell proliferation; central nervous system development; chromosome organization and biogenesis; DNA ligation; DNA ligation during DNA recombination; DNA ligation during DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; lagging strand elongation; negative regulation of neuron apoptosis; neuron apoptosis; nucleotide-excision repair, DNA gap filling; positive regulation of fibroblast proliferation; positive regulation of neurogenesis; pro-B cell differentiation; response to gamma radiation; response to X-ray; single strand break repair; somatic stem cell maintenance; T cell differentiation in the thymus; T cell receptor V(D)J recombination; V(D)J recombination

Disease: Lig4 Syndrome; Myeloma, Multiple
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Research Articles on LIG4

1. LIG4 is highly upregulated in human colorectal cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with LIG4 cdna clone

 Products by Pathway  Pathway Diagram
 2-LTR Circle Formation Pathway antibodies  2-LTR Circle Formation Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Integration Of Provirus Pathway antibodies  Integration Of Provirus Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram

Diseases associated with LIG4 cdna clone

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >42 publications with LIG4 and Neoplasms
 LIG4 Syndrome Antibodies  >27 publications with LIG4 and LIG4 Syndrome
 Nervous System Diseases Antibodies  >10 publications with LIG4 and Nervous System Diseases
 Lymphoma Antibodies  >10 publications with LIG4 and Lymphoma
 Respiratory Tract Diseases Antibodies  >6 publications with LIG4 and Respiratory Tract Diseases
 Neoplasms, Experimental Antibodies  >6 publications with LIG4 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >5 publications with LIG4 and Drug Toxicity
 Carcinoma, Squamous Cell Antibodies  >5 publications with LIG4 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >4 publications with LIG4 and Lung Neoplasms
 Disease Models, Animal Antibodies  >4 publications with LIG4 and Disease Models, Animal

Organs/Tissues associated with LIG4 cdna clone

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with LIG4 and Blood
 Brain Antibodies  >8 publications with LIG4 and Brain
 Bone Antibodies  >7 publications with LIG4 and Bone
 Skin Antibodies  >4 publications with LIG4 and Skin
 Prostate Antibodies  >3 publications with LIG4 and Prostate
 Thymus Antibodies  >3 publications with LIG4 and Thymus
 Muscle Antibodies  >2 publications with LIG4 and Muscle
 Pharynx Antibodies  >2 publications with LIG4 and Pharynx
 Kidney Antibodies  >1 publications with LIG4 and Kidney
 Testis Antibodies  >1 publications with LIG4 and Testis
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