Human NAE1 cDNA Clone Expression Plasmid – Cat# MBS1270405

NAE1 cDNA Clone – NEDD8 Activating Enzyme E1 Subunit 1 (Human)

Name: NAE1 cDNA Clone; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1270405
Price: 235 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1270405

MBS1270405 is a cDNA clone targeting NAE1. More details

$235 / 0.01 mg Plasmid + 0.2 mL Glycerol-Stock +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NAE1, cDNA Clone

Full Product Name

NAE1 cDNA Clone

Product Gene Name

NAE1 cdna clone

Sequence

atggcgcagc tgggaaagct gctcaaggag cagaagtacg accggcagct gaggttgtgg ggtgatcatg ggcaagaggc tttagaatct gctcatgttt gcctaataaa tgcaacagcc acaggaactg aaattcttaa aaacttggta ctaccaggta ttggttcgtt tacaattatt gatggaaatc aggtcagcgg agaagatgct ggaaacaatt tcttccttca aagaagcagt atcggcaaga accgagctga agctgccatg gaattcttac aagaattaaa tagcgatgtc tctggaagtt ttgtggaaga gagtccagaa aaccttctag acaatgatcc ctcatttttc tgtaggttta ctgttgtagt tgcaactcag cttcctgaaa gcacttcact acgcttagca gatgtcctct ggaattccca gattcctctt ttgatctgta ggacatatgg actagttggt tatatgagga tcattataaa agaacatcca gtaatagaat ctcatccaga taatgcatta gaggatctac gactagataa gccatttcct gaactgagag aacattttca gtcctatgat ttggatcata tggaaaaaaa ggaccacagt catactccat ggattgtgat catagctaaa tatttagcac agtggtatag tgaaacaaat ggacgaatac ctaaaacgta taaagaaaaa gaggacttca gagatttgat tagacaagga attctaaaaa atgaaaatgg ggctccagaa gatgaagaga attttgaaga agctattaaa aatgtgaaca cagcactaaa tacaactcag atcccaagca gtattgaaga tatatttaat gatgatcgct gcataaatat caccaaacag actccatcat tttggatttt agctcgtgcc ttaaaggaat ttgtggccaa agagggtcaa ggaaatttac ctgttcgagg cacaattcct gatatgattg cagattcagg caaatatata aaactgcaaa acgtttaccg tgaaaaagca aagaaagatg ctgccgctgt gggtaatcat gttgccaaat tgctgcagtc cattggccag gcaccagagt ccatttcaga gaaagaatta aaattactct gcagcaattc tgcatttctt cgagtggtaa gatgtcgatc cttagctgaa gaatatggtt tggatacaat taacaaggat gaaattattt ctagcatgga caatccagat aatgaaatag tgttgtactt aatgttacgg gctgttgata gatttcataa acaacagggt agatatccag gagtatctaa ctatcaagtt gaagaagata taggaaagtt gaagtcttgt ctcactggct tccttcagga atatggttta tctgtaatgg tgaaagatga ttatgtccac gaattttgcc gatatggagc tgctgagcca cataccattg ctgcattctt ggggggagct gctgctcaag aggtcatcaa aataatcacc aaacaatttg taatttttaa taatacttac atttacagtg gcatgtcaca aacttcagca actttccagt tgtag

Molecular Weight

60,517 Da

OMIM

603385

Vector

pENTR223.1

Clone Sequence Report

Provided with product shipment

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NAE1 cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NAE1. It may not necessarily be applicable to this product.

NCBI GI #

38197166

NCBI GeneID

8883

NCBI Accession #

BC000480

UniProt Secondary Accession #

A6NCK0; A6NFN4; A8MU28; B2R700; B3KUP9

UniProt Related Accession #

Q13564

NCBI Official Full Name

Homo sapiens NEDD8 activating enzyme E1 subunit 1, mRNA

NCBI Official Synonym Full Names

NEDD8 activating enzyme E1 subunit 1

NCBI Official Symbol

NAE1

NCBI Official Synonym Symbols

HPP1; ula-1; APPBP1; A-116A10.1

UniProt Protein Name

NEDD8-activating enzyme E1 regulatory subunit

UniProt Synonym Protein Names

Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1

Protein Family

NEDD8-activating enzyme E1 regulatory

UniProt Gene Name

NAE1

UniProt Synonym Gene Names

APPBP1; APP-BP1

UniProt Entry Name

ULA1_HUMAN

NCBI Protein Information

NEDD8-activating enzyme E1 regulatory subunit

NCBI Summary for NAE1

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for NAE1

APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding

Biological Process: mitotic cell cycle DNA replication checkpoint; neuron apoptosis; protein neddylation; regulation of apoptosis; regulation of neuron apoptosis; signal transduction

Research Articles on NAE1

1. E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NAE1 cdna clone

Products by Pathway	Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimers Disease Pathway antibodies	Alzheimers Disease Pathway Diagram

Diseases associated with NAE1 cdna clone

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>1 publications with NAE1 and Cardiovascular Diseases
Neoplasms Antibodies	>1 publications with NAE1 and Neoplasms
Breast Neoplasms Antibodies	>1 publications with NAE1 and Breast Neoplasms

Organs/Tissues associated with NAE1 cdna clone

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>2 publications with NAE1 and Kidney
Muscle Antibodies	>1 publications with NAE1 and Muscle
Heart Antibodies	>1 publications with NAE1 and Heart
Skin Antibodies	>1 publications with NAE1 and Skin
Brain Antibodies	>1 publications with NAE1 and Brain