Human PAM cDNA Clone Expression Plasmid – Cat# MBS1273068

PAM cDNA Clone – Peptidylglycine Alpha-Amidating Monooxygenase (Human)

Name: PAM cDNA Clone; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1273068
Price: 965 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1273068

MBS1273068 is a cDNA clone targeting PAM. More details

$965 / 0.01 mg Plasmid + 0.2 mL Glycerol-Stock +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PAM, cDNA Clone

Full Product Name

PAM cDNA Clone

Product Gene Name

PAM cdna clone

Sequence

atggctggcc gcgtccctag cctgctagtt ctccttgttt ttccaagcag ctgtttggct ttccgaagcc cactttctgt ctttaagagg tttaaagaaa ctaccagacc attttccaat gaatgtcttg gtaccaccag acccgtagtt cctattgatt catcagattt tgcattggat attcgcatgc ctggggttac acctaaacag tccgatacat acttctgcat gtctatgcga ataccagtgg atgaggaagc cttcgtgatt gacttcaagc ctcgagccag catggatact gtccatcaca tgttactttt tggatgcaat atgccttcat ccactggaag ttactggttt tgtgatgaag gaacctgtac agataaagcc aatattctgt atgcctgggc gagaaatgct ccccctaccc ggctccccaa aggtgttgga ttcagagttg gaggagagac tggaagtaaa tactttgtac tacaggtaca ctatggggat attagtgctt ttagagataa taacaaggac tgttctggtg tgtccttaca cctcacacgt ctgccacagc ctttaattgc tggcatgtac cttatgatgt ctgttgacac tgttatccca gcaggagaaa aagtggtgaa ttctgacatt tcatgccatt ataaaaatta tccaatgcat gtctttgcct atagagttca cactcaccat ttaggtaagg tagtaagtgg atacagagta agaaatggac agtggacact gattggacgg cagagccctc agctgccaca ggctttctac cctgtggggc atccagttga tgtaagtttt ggtgacctac tggctgcaag atgtgtattc actggtgaag gaaggacaga agccacacac attggtggca cgtctagtga tgaaatgtgc aacttataca ttatgtatta catggaagcc aagcatgcag tttctttcat gacctgtacc cagaatgtag ctccagatat gttcagaacc ataccaccag aggccaacat tccaattccc gtgaagtctg atatggttat gatgcatgaa catcataaag aaacagaata taaagataag attcctttac tacagcagcc aaaacgagaa gaagaagaag tgttagacca ggatttccac atggaagagg cactggattg gcctggagta tacttgttac caggccaggt ttctggggtg gctctagacc ctaagaataa cctggtgatt ttccacagag gtgaccatgt ctgggatgga aactcgtttg acagcaagtt tgtttaccag caaataggac tcggaccaat tgaagaagac actattcttg tcatagatcc aaataatgct gcagtactcc agtccagtgg aaaaaatctg ttttacttgc cacatggctt gagtatagat aaagatggga attattgggt cacagacgtg gctctccatc aggtgttcaa actggatcca aacaataaag aaggccctgt attaatcctg ggaaggagca tgcaaccagg cagtgaccag aatcacttct gtcaacccac tgatgtggct gtggatccag gcactggagc catttatgta tcagatggtt actgcaacag caggattgtg cagttttcac caagtggaaa gttcatcaca cagtggggag aagagtcttc agggagcagt cctctgccag gccagttcac tgttcctcac agcttggctc ttgtgcctct tttgggccaa ttatgtgtgg cagaccggga aaatggtcgg atccagtgtt ttaaaactga caccaaagaa tttgtgagag agattaagca ttcatcattt ggaagaaatg tatttgcaat ttcatatata ccaggcttgc tctttgcagt gaatgggaag cctcattttg gggaccaaga acctgtacaa ggatttgtga tgaacttttc caatggggaa attatcgaca tcttcaagcc agtgcgcaag cactttgata tgcctcatga tattgttgca tctgaagatg ggactgtgta cattggagat gctcatacca acaccgtgtg gaagttcacc ttgactgaga aattggaaca tcgatcagtt aaaaaggctg gcattgaggt ccaggaaatc aaagaagccg aggcagttgt tgaaaccaaa atggagaaca aacccacctc ctcagaattg cagaagatgc aagagaaaca gaaactgatc aaagagccag gctcgggagt gcctgttgtt ctcattacaa cccttctggt tattccggtg gttgtcctgc tggccattgc catatttatt cggtggaaaa aatcaagggc ctttggagat tctgaacaca aactcgagac gagttcagga agagtactgg gaagatttag aggaaaggga agtggaggct taaaccttgg taatttcttt gcaagccgta agggctacag tcgaaaaggg tttgaccggc ttagcactga gggcagtgac caagagaaag aggatgatgg aagtgaatca gaagaggagt attcagcacc tctgcctgcg ctcgcacctt cctcctcctg a

Molecular Weight

106,276 Da

OMIM

170270

Vector

Please Inquire

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PAM cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PAM. It may not necessarily be applicable to this product.

NCBI GI #

17390285

NCBI GeneID

5066

NCBI Accession #

BC018127

UniProt Secondary Accession #

O43211; O95080; Q16252; Q16253; Q54A45; Q86U53; Q8WVC7; Q9UCG0; A6NMR0; A8K293

UniProt Related Accession #

P19021

NCBI Official Full Name

Homo sapiens peptidylglycine alpha-amidating monooxygenase, mRNA

NCBI Official Synonym Full Names

peptidylglycine alpha-amidating monooxygenase

NCBI Official Symbol

PAM

NCBI Official Synonym Symbols

PAL; PHM

UniProt Protein Name

Peptidyl-glycine alpha-amidating monooxygenase

UniProt Synonym Protein Names

Peptidylglycine alpha-hydroxylating monooxygenase (EC:1.14.17.3); PHMPeptidyl-alpha-hydroxyglycine alpha-amidating lyase (EC:4.3.2.5)Alternative name(s):Peptidylamidoglycolate lyase; PAL

Protein Family

Peptidyl-glycine alpha-amidating monooxygenase

UniProt Gene Name

PAM

UniProt Synonym Gene Names

PAM; PHM; PAL

UniProt Entry Name

AMD_HUMAN

NCBI Protein Information

peptidyl-glycine alpha-amidating monooxygenase

NCBI Summary for PAM

This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

UniProt Comments for PAM

PAM: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.17.3; EC 4.3.2.5; Membrane protein, integral; Vesicle; Lyase; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q14-q21

Cellular Component: membrane

Molecular Function: peptidylamidoglycolate lyase activity; peptidylglycine monooxygenase activity; protein binding; zinc ion binding

Biological Process: peptide amidation

Research Articles on PAM

1. Data suggest that His108 and a substrate molecule are involved in the reductive pathway while His172 and Tyr79 are important in the catalytic pathway in the copper-centered electron transfer catalyzed by peptidylglycine monooxygenase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PAM cdna clone

Disease Name	Pubmed Publications
Neoplasms Antibodies	>816 publications with PAM and Neoplasms
Nervous System Diseases Antibodies	>438 publications with PAM and Nervous System Diseases
Drug Toxicity Antibodies	>358 publications with PAM and Drug Toxicity
Poisoning Antibodies	>245 publications with PAM and Poisoning
Brain Diseases Antibodies	>235 publications with PAM and Brain Diseases
Inflammation Antibodies	>211 publications with PAM and Inflammation
Necrosis Antibodies	>193 publications with PAM and Necrosis
Disease Models, Animal Antibodies	>157 publications with PAM and Disease Models, Animal
Breast Neoplasms Antibodies	>129 publications with PAM and Breast Neoplasms
Heart Diseases Antibodies	>125 publications with PAM and Heart Diseases

Organs/Tissues associated with PAM cdna clone

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1121 publications with PAM and Blood
Brain Antibodies	>432 publications with PAM and Brain
Lung Antibodies	>379 publications with PAM and Lung
Bone Antibodies	>277 publications with PAM and Bone
Heart Antibodies	>213 publications with PAM and Heart
Skin Antibodies	>206 publications with PAM and Skin
Vascular Antibodies	>203 publications with PAM and Vascular
Muscle Antibodies	>185 publications with PAM and Muscle
Nerve Antibodies	>175 publications with PAM and Nerve
Kidney Antibodies	>161 publications with PAM and Kidney