POLR3H cDNA Clone – DNA-Directed RNA Polymerase III Subunit RPC8 Isoform a

POLR3H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; centrosome; DNA-directed RNA polymerase III complex; intracellular membrane-bound organelle; cytosol

Molecular Function: DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; termination of RNA polymerase III transcription; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter