Human SPAM1 cDNA Clone Expression Plasmid – Cat# MBS1275585

SPAM1 cDNA Clone – Sperm Adhesion Molecule 1 (Ph-20 Hyaluronidase, Zona Pellucida Binding) (Human)

Name: SPAM1 cDNA Clone; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1275585
Price: 235 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1275585

MBS1275585 is a cDNA clone targeting SPAM1. More details

$235 / 0.01 mg Plasmid + 0.2 mL Glycerol-Stock +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SPAM1, cDNA Clone

Full Product Name

SPAM1 cDNA Clone

Product Gene Name

SPAM1 cdna clone

Sequence

atgggagtgc taaaattcaa gcacatcttt ttcagaagct ttgttaaatc aagtggagta tcccagatag ttttcacctt ccttctgatt ccatgttgct tgactctgaa tttcagagca cctcctgtta ttccaaatgt gcctttcctc tgggcctgga atgccccaag tgaattttgt cttggaaaat ttgatgagcc actagatatg agcctcttct ctttcatagg aagcccccga ataaacgcca ccgggcaagg tgttacaata ttttatgttg atagacttgg ctactatcct tacatagatt caatcacagg agtaactgtg aatggaggaa tcccccagaa gatttcctta caagaccatc tggacaaagc taagaaagac attacatttt atatgccagt agacaatttg ggaatggctg ttattgactg ggaagaatgg agacccactt gggcaagaaa ctggaaacct aaagatgttt acaagaatag gtctattgaa ttggttcagc aacaaaatgt acaacttagt ctcacagagg ccactgagaa agcaaaacaa gaatttgaaa aagcagggaa ggatttcctg gtagagacta taaaattggg aaaattactt cggccaaatc acttgtgggg ttattatctt tttccggatt gttacaacca tcactataag aaacccggtt acaatggaag ttgcttcaat gtagaaataa aaagaaatga tgatctcagc tggttgtgga atgaaagcac tgctctttac ccatccattt atttgaacac tcagcagtct cctgtagctg ctacactcta tgtgcgcaat cgagttcggg aagccatcag agtttccaaa atacctgatg caaaaagtcc acttccggtt tttgcatata cccgcatagt ttttactgat caagttttga aattcctttc tcaagatgaa cttgtgtata catttggcga aactgttgct ctgggtgctt ctggaattgt aatatgggga accctcagta taatgcgaag tatgaaatct tgcttgctcc tagacaatta catggagact atactgaatc cttacataat caacgtcaca ctagcagcca aaatgtgtag ccaagtgctt tgccaggagc aaggagtgtg tataaggaaa aactggaatt caagtgacta tcttcacctc aacccagata attttgctat tcaacttgag aaaggtggaa agttcacagt acgtggaaaa ccgacacttg aagacctgga gcaattttct gaaaaatttt attgcagctg ttatagcacc ttgagttgta aggagaaagc tgatgtaaaa gacactgatg ctgttgatgt gtgtattgct gatggtgtct gtatagatgc ttttctaaaa cctcccatgg agacagaaga acctcaaatt ttctacaatg cttcaccctc cacactatct gccacaatgt tcatttggag gctggaagtc tgggatcaag gtattagcag aattggtttc ttctga

Molecular Weight

58,395 Da

OMIM

600930

Vector

pENTR223.1

Clone Sequence Report

Provided with product shipment

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SPAM1 cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPAM1. It may not necessarily be applicable to this product.

NCBI GI #

20072877

NCBI GeneID

6677

NCBI Accession #

BC026163

UniProt Secondary Accession #

Q8TC30

UniProt Related Accession #

P38567

NCBI Official Full Name

Homo sapiens sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), mRNA

NCBI Official Synonym Full Names

sperm adhesion molecule 1

NCBI Official Symbol

SPAM1

NCBI Official Synonym Symbols

HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n

UniProt Protein Name

Hyaluronidase PH-20

UniProt Synonym Protein Names

Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20

Protein Family

Hyaluronidase

UniProt Gene Name

SPAM1

UniProt Synonym Gene Names

HYAL3; PH20; Hyal-PH20

UniProt Entry Name

HYALP_HUMAN

NCBI Protein Information

hyaluronidase PH-20

NCBI Summary for SPAM1

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

UniProt Comments for SPAM1

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell surface; EC 3.2.1.35; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Biological Process: binding of sperm to zona pellucida; sperm-egg recognition

Research Articles on SPAM1

1. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SPAM1 cdna clone

Products by Pathway	Pathway Diagram
Chondroitin Sulfate Degradation Pathway antibodies	Chondroitin Sulfate Degradation Pathway Diagram
Chondroitin Sulfate Degradation Pathway antibodies	Chondroitin Sulfate Degradation Pathway Diagram
Dermatan Sulfate Degradation Pathway antibodies	Dermatan Sulfate Degradation Pathway Diagram
Dermatan Sulfate Degradation Pathway antibodies	Dermatan Sulfate Degradation Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram

Diseases associated with SPAM1 cdna clone

Disease Name	Pubmed Publications
Necrosis Antibodies	>1 publications with SPAM1 and Necrosis
Liver Neoplasms Antibodies	>1 publications with SPAM1 and Liver Neoplasms

Organs/Tissues associated with SPAM1 cdna clone

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>25 publications with SPAM1 and Testis
Brain Antibodies	>1 publications with SPAM1 and Brain