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AXL Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8116400

Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in… More details

$315 / 0.3 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
AXL, Cell Lysate
Full Product Name
Mouse AXL HEK293 Overexpression Lysate
Product Gene Name
AXL cell lysate
Product Synonym Names
Mouse Axl Kinase HEK293 Cell Lysate (WB positive control); AXL receptor tyrosine kinase; Mouse AI323647 Overexpression Lysate; Mouse Ark Overexpression Lysate; Mouse Tyro7 Overexpression Lysate; Mouse Ufo Overexpression Lysate
Sequence
Met1-Pro44 3
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
98,191 Da
Sequence Positions
435aa
Chromosome Location
Chromosome: 7; NC_000073.6 (25756500..25788733, complement). Location: 7 A3-B1; 7 14.02 cM
3D Structure
ModBase 3D Structure for Q00993
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain (Met 1-Pro 443) of mouse AXL (NP_033491.2) precursor was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Product Categories/Family for AXL cell lysate

Receptor Tyrosine Kinases (RTKs)
Applications Tested/Suitable for AXL cell lysate
Western Blot (WB)
Application Notes for AXL cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for AXL cell lysate

Weinger JG, et al. (2011) Loss of the receptor tyrosine kinase Axl leads to enhanced inflammation in the CNS and delayed removal of myelin debris during Experimental Autoimmune Encephalomyelitis. J Neuroinflammation. 8: 49.Linger RM, et al. (2010) Taking aim at Mer and Axl receptor tyrosine kinases as novel therapeutic targets in solid tumors. Expert Opin Ther Targets. 14(10): 1073-90.Cavet ME, et al. (2010) Gas6-Axl pathway: the role of redox-dependent association of Axl with nonmuscle myosin IIB. Hypertension. 56(1): 105-11.Rankin EB, et al. (2010) AXL is an essential factor and therapeutic target for metastatic ovarian cancer. Cancer Res. 70(19): 7570-9.

Other Notes

Small volumes of AXL cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AXL purchase

MBS8116400 is a cell lysate targeting AXL. Also known as Mouse AXL HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for AXL. It may not necessarily be applicable to this product.

NCBI GI #
31542164
NCBI GeneID
26362
NCBI Accession #
NP_033491.2
UniProt Primary Accession #
Q00993
UniProt Secondary Accession #
Q80YQ3
UniProt Related Accession #
Q00993
GenBank Nucleotide #
NM_009465.4
NCBI Official Full Name
tyrosine-protein kinase receptor UFO isoform 1
NCBI Official Synonym Full Names
AXL receptor tyrosine kinase
NCBI Official Symbol
Axl
NCBI Official Synonym Symbols
Ark; Ufo; Tyro7; AI323647
UniProt Protein Name
Tyrosine-protein kinase receptor UFO
UniProt Synonym Protein Names
Adhesion-related kinase
Protein Family
Tyrosine-protein kinase receptor
UniProt Gene Name
Axl
UniProt Synonym Gene Names
Ark; Ufo
UniProt Entry Name
UFO_MOUSE
NCBI Protein Information
tyrosine-protein kinase receptor UFO; ufo oncogene homolog; adhesion-related kinase

UniProt Comments for AXL

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Kinase, protein; TK group; Axl family

Cellular Component: extracellular space; cell surface; membrane; cell; integral to membrane; plasma membrane; intracellular

Molecular Function: transferase activity; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; phosphoinositide 3-kinase binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; neuron migration; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; negative regulation of neuron apoptosis; inflammatory response; cell differentiation; secretion by cell; forebrain cell migration; negative regulation of lymphocyte activation; platelet activation; immune system process; vagina development; negative regulation of tumor necrosis factor production; phagocytosis; positive regulation of protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; spermatogenesis; blood vessel remodeling; positive regulation of cytokine and chemokine mediated signaling pathway; phosphorylation; apoptotic cell clearance; negative regulation of apoptosis
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Research Articles on AXL

1. Chronic systemic inflammation and autoimmune disorders in the Tyro3, Axl and Mertk knockout mice cause neuronal damage and death.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AXL cell lysate

 Reference Product  PubMed Publications
 Gas6 cell lysate  >338 publications with AXL and Gas6
 Trp53 cell lysate  >10 publications with AXL and Trp53
 Snai1 cell lysate  >7 publications with AXL and Snai1
 Anxa2 cell lysate  >2 publications with AXL and Anxa2
 Ranbp9 cell lysate  >1 publications with AXL and Ranbp9

Pathways associated with AXL cell lysate

 Products by Pathway  Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By VEGF Pathway antibodies  Signaling By VEGF Pathway Diagram
 VEGFA-VEGFR2 Pathway antibodies  VEGFA-VEGFR2 Pathway Diagram

Diseases associated with AXL cell lysate

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >137 publications with AXL and Carcinoma
 Inflammation Antibodies  >85 publications with AXL and Inflammation
 Lung Neoplasms Antibodies  >64 publications with AXL and Lung Neoplasms
 Adenocarcinoma Antibodies  >63 publications with AXL and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >57 publications with AXL and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >50 publications with AXL and Breast Neoplasms
 Disease Models, Animal Antibodies  >48 publications with AXL and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >38 publications with AXL and Neoplasms, Experimental
 Kidney Diseases Antibodies  >37 publications with AXL and Kidney Diseases
 Necrosis Antibodies  >36 publications with AXL and Necrosis

Organs/Tissues associated with AXL cell lysate

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >162 publications with AXL and Blood
 Lung Antibodies  >103 publications with AXL and Lung
 Brain Antibodies  >70 publications with AXL and Brain
 Eye Antibodies  >69 publications with AXL and Eye
 Bone Antibodies  >58 publications with AXL and Bone
 Muscle Antibodies  >52 publications with AXL and Muscle
 Kidney Antibodies  >52 publications with AXL and Kidney
 Liver Antibodies  >45 publications with AXL and Liver
 Bone Marrow Antibodies  >41 publications with AXL and Bone Marrow
 Heart Antibodies  >24 publications with AXL and Heart
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