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CD300LF Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8115612

This Human CD300f/CD300LF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD300f/CD300LF protein from the overexpression lysate was verified. More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD300f/CD300LF, Cell Lysate
Full Product Name
Human CD300f/CD300LF HEK293 Overexpression Lysate
Product Gene Name
Product Synonym Names
Human CD300LF/LMIR3 HEK293 Cell Lysate (WB positive control); CD300 molecule-like family member f; Human CD300f Overexpression Lysate; Human CLM-1 Overexpression Lysate; Human CLM1 Overexpression Lysate; Human IgSF13 Overexpression Lysate; Human IREM-1 Overexpression Lysate; Human IREM1 Overexpression Lysate; Human LMIR3 Overexpression Lysate; Human NKIR Overexpression Lysate
Sequence
Met1-Leu15 5
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
32,335 Da
Sequence Positions
374aa
Chromosome Location
Chromosome: 17; NC_000017.10 (72690452..72709108, complement). Location: 17q25.1
OMIM
609807
3D Structure
ModBase 3D Structure for Q8TDQ1
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CD300LF (NP_620587.2) (Met1-Leu155) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for CD300LF cell lysate
Western Blot (WB)
Application Notes for CD300LF cell lysate
WB: Use at an assay dependent dilution.

Other Notes

Small volumes of CD300LF cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD300LF purchase

MBS8115612 is a cell lysate targeting CD300LF. Also known as Human CD300f/CD300LF HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD300LF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CMRF35-like molecule 1
NCBI Official Synonym Full Names
CD300 molecule-like family member f
NCBI Official Symbol
CD300LF
NCBI Official Synonym Symbols
CLM1; NKIR; CLM-1; IREM1; CD300f; IREM-1; IgSF13
UniProt Protein Name
CMRF35-like molecule 1
UniProt Synonym Protein Names
CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor
Protein Family
UniProt Gene Name
CD300LF
UniProt Synonym Gene Names
CD300F; CLM1; IGSF13; IREM1; NKIR; CLM-1; IREM-1; IgSF13
UniProt Entry Name
CLM1_HUMAN
NCBI Protein Information
CMRF35-like molecule 1; NK inhibitory receptor; inhibitory receptor IREM1; CD300 antigen like family member F; CD300 antigen-like family member F; immunoglobin superfamily member 13; CD300 molecule like family member f; immunoglobulin superfamily member 1

NCBI Summary for CD300LF

CD300LF is an inhibitory receptor of the Ig superfamily expressed on myeloid cells. It mediates negative regulatory signals by recruiting SHP1 (PTPN6; MIM 176883) or SHIP (INPP5D; MIM 601582) (Sui et al., 2004 [PubMed 15184070]; Alvarez-Errico et al., 2004 [PubMed 15549731]).[supplied by OMIM, Mar 2008]
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UniProt Comments for CD300LF

IREM-1: Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. Belongs to the CD300 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: integral to membrane; plasma membrane

Biological Process: immune system process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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