DKKL1 Cell Lysate – Tissue Lysate

Name: DKKL1 Cell Lysate; Tissue Lysate; MyBioSource
Brand: MyBioSource
SKU: MBS8114178
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8114178

Dickkopf-like 1 (DKKL1) or soggy 1, is a glycoprotein unique to mammals that is expressed primarily in developing spermatocytes and localized in the acrosome of mature sperm. It is also expressed in the trophectoderm/placental lineage. This glycoprotein is… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DKKL1, Cell Lysate

Full Product Name

Human DKKL1 HEK293 Overexpression Lysate

Product Gene Name

DKKL1 cell lysate

Product Synonym Names

Human DKKL1/Soggy-1/SGY-1 HEK293 Cell Lysate (WB positive control); dickkopf-like 1; Human CT34 Overexpression Lysate; Human SGY Overexpression Lysate; Human SGY-1 Overexpression Lysate; Human SGY1 Overexpression Lysate

Sequence

Ala31-Leu2 42

Host

HEK293 Cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Molecular Weight

27,007 Da

Sequence Positions

472aa

3D Structure

ModBase 3D Structure for Q9UK85

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the secreted form of human DKKL1 (Q9UK85) (Ala 31-Leu 242) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Applications Tested/Suitable for DKKL1 cell lysate
Western Blot (WB)

Application Notes for DKKL1 cell lysate
WB: Use at an assay dependent dilution.

Related Product Information for DKKL1 cell lysate
Dickkopf-like 1 (DKKL1) or soggy 1, is a glycoprotein unique to mammals that is expressed primarily in developing spermatocytes and localized in the acrosome of mature sperm. It is also expressed in the trophectoderm/placental lineage. This glycoprotein is secreted by postmeiotic male germ cells. DKKL1 is a member of the Dickkopf (DKK) family, a group of proteins that are characterized as secreted antagonists of Wnt signal transduction proteins. In mammals, embryos lacking DKKL1 protein developed into viable, fertile adults. DKKL1, either directly or indirectly, facilitates the ability of sperm to penetrate the zona pellucid. DKKL1 is related to the sperm apoptotic procession. Molecular analyses identified the Fas death ligand (FasL) as a target for DkkL1 pro-apoptotic activity in adult mice. DKKL1 is considered as a negative regulator of adult testic homeostasis and identifies a novel, DKKL1/FasL-dependent, regulation that specifically controls the number of postpubertal spermatocytes.

This Human DKKL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DKKL1 protein from the overexpression lysate was verified.

Product References and Citations for DKKL1 cell lysate
Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators. Oncogene. 25 (57): 7469-81.Kohn MJ, et al. (2010) The Acrosomal Protein Dickkopf-Like 1 (DKKL1) Facilitates Sperm Penetration Of The Zona Pellucida. Fertil Steril. 93 (5): 1533-7.KOHN MJ, et al. (2005) DKKL1 (Soggy), A Dickkopf Family Member, Localizes to the Acrosome During Mammalian Spermatogenesis. Mol Reprod Dev. 71 (4): 516-22.

Other Notes
Small volumes of DKKL1 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DKKL1 purchase
MBS8114178 is a cell lysate targeting DKKL1. Also known as Human DKKL1 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for DKKL1. It may not necessarily be applicable to this product.

NCBI GI #
308818218
NCBI GeneID
27120
NCBI Accession #
NP_001184230.1

UniProt Primary Accession #
Q9UK85
UniProt Related Accession #
Q9UK85
GenBank Nucleotide #
NM_001197301.1

NCBI Official Full Name
dickkopf-like protein 1 isoform 2
NCBI Official Synonym Full Names
dickkopf like acrosomal protein 1
NCBI Official Symbol
DKKL1
NCBI Official Synonym Symbols
SGY; CT34; SGY1; SGY-1

UniProt Protein Name
Dickkopf-like protein 1
UniProt Synonym Protein Names
Cancer/testis antigen 34; CT34; Protein soggy-1; SGY-1
Protein Family
Dickkopf-like protein
UniProt Gene Name
DKKL1
UniProt Synonym Gene Names
CT34; SGY-1

NCBI Protein Information
dickkopf-like protein 1

NCBI Summary for DKKL1

The dickkopf protein family interacts with the Wnt signaling pathway and its members are characterized by two conserved cysteine-rich domains. This gene encodes a secreted protein that has low sequence similarity to the dickkopf-3 protein. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
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UniProt Comments for DKKL1

Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promotes spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduces testosterone synthesis in Leydig cells. Is not essential either for development or fertility.

Research Articles on DKKL1

1. DKKL was overexpressed in colorectal cancer tissue compared with healthy colon. DKKL expression positively correlated with colorectal cancer progression.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DKKL1 cell lysate

Reference Product PubMed Publications

DKK1 cell lysate >2 publications with DKKL1 and DKK1

Diseases associated with DKKL1 cell lysate

Disease Name Pubmed Publications

Infertility, Female Antibodies >3 publications with DKKL1 and Infertility, Female

Congenital Abnormalities Antibodies >1 publications with DKKL1 and Congenital Abnormalities

Weight Gain Antibodies >1 publications with DKKL1 and Weight Gain

Urogenital Abnormalities Antibodies >1 publications with DKKL1 and Urogenital Abnormalities

Organs/Tissues associated with DKKL1 cell lysate

Organ/Tissue Name Pubmed Publications

Testis Antibodies >6 publications with DKKL1 and Testis

Mixed Antibodies >2 publications with DKKL1 and Mixed

Eye Antibodies >1 publications with DKKL1 and Eye

Brain Antibodies >1 publications with DKKL1 and Brain

Disease Name	Pubmed Publications
Infertility, Female Antibodies	>3 publications with DKKL1 and Infertility, Female
Congenital Abnormalities Antibodies	>1 publications with DKKL1 and Congenital Abnormalities
Weight Gain Antibodies	>1 publications with DKKL1 and Weight Gain
Urogenital Abnormalities Antibodies	>1 publications with DKKL1 and Urogenital Abnormalities

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>6 publications with DKKL1 and Testis
Mixed Antibodies	>2 publications with DKKL1 and Mixed
Eye Antibodies	>1 publications with DKKL1 and Eye
Brain Antibodies	>1 publications with DKKL1 and Brain