• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

EPOR Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8116311

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO… More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EPOR, Cell Lysate
Full Product Name
Mouse EPOR HEK293 Overexpression Lysate
Product Gene Name
EPOR cell lysate
Product Synonym Names
Mouse EPOR HEK293 Cell Lysate (WB positive control); erythropoietin receptor
Sequence
Met1-Pro24 9
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
55,194 Da
Sequence Positions
236aa
3D Structure
ModBase 3D Structure for P14753
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for EPOR cell lysate
Western Blot (WB)
Application Notes for EPOR cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for EPOR cell lysate

Divoky V, et al. (2002) Mouse surviving solely on human erythropoietin receptor (EpoR): model of human EpoR-linked disease. Blood 99(10): 3873-4.Carruthers SG. (2009) A truncated erythropoietin receptor EPOR-T is associated with hypertension susceptibility. Clin Pharmacol Ther. 86(2): 134-6.Baltaziak M, et al. (2009) Relationships of P53 and Bak with EPO and EPOR in human colorectal cancer. Anticancer Res. 29(10):4151-6.

Other Notes

Small volumes of EPOR cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPOR purchase

MBS8116311 is a cell lysate targeting EPOR. Also known as Mouse EPOR HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for EPOR. It may not necessarily be applicable to this product.

NCBI GI #
116292186
NCBI GeneID
13857
NCBI Accession #
NP_034279.3
UniProt Primary Accession #
P14753
UniProt Secondary Accession #
Q63852
GenBank Nucleotide #
NM_010149.3
NCBI Official Full Name
erythropoietin receptor
UniProt Protein Name
Erythropoietin receptor
Protein Family
Erythropoietin receptor
UniProt Gene Name
Epor
UniProt Synonym Gene Names
EPO-R
UniProt Entry Name
EPOR_MOUSE

UniProt Comments for EPOR

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: membrane; cell; extracellular region; integral to membrane; plasma membrane; cytosol

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity; erythropoietin receptor activity

Biological Process: heart morphogenesis; elevation of cytosolic calcium ion concentration; embryonic development; viral reproduction; heart development; cardiac muscle morphogensis; ventricular cardiac muscle morphogenesis; negative regulation of neuron apoptosis; brain development; decidualization; signal transduction
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EPOR cell lysate

 Reference Product  PubMed Publications
 EPO cell lysate  >616 publications with EPOR and EPO
 JAK2 cell lysate  >162 publications with EPOR and JAK2
 KITLG cell lysate  >93 publications with EPOR and KITLG
 KIT cell lysate  >45 publications with EPOR and KIT
 MAPK3 cell lysate  >15 publications with EPOR and MAPK3
 PTPN6 cell lysate  >13 publications with EPOR and PTPN6
 MAPK1 cell lysate  >13 publications with EPOR and MAPK1
 STAT5A cell lysate  >12 publications with EPOR and STAT5A
 PTPN11 cell lysate  >10 publications with EPOR and PTPN11
 GRB2 cell lysate  >9 publications with EPOR and GRB2

Pathways associated with EPOR cell lysate

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram

Diseases associated with EPOR cell lysate

 Disease Name  Pubmed Publications
 Anemia Antibodies  >164 publications with EPOR and Anemia
 Polycythemia Antibodies  >78 publications with EPOR and Polycythemia
 Cardiovascular Diseases Antibodies  >70 publications with EPOR and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >59 publications with EPOR and Disease Models, Animal
 Kidney Diseases Antibodies  >36 publications with EPOR and Kidney Diseases
 Breast Neoplasms Antibodies  >32 publications with EPOR and Breast Neoplasms
 Inflammation Antibodies  >28 publications with EPOR and Inflammation
 Necrosis Antibodies  >28 publications with EPOR and Necrosis
 Adenocarcinoma Antibodies  >28 publications with EPOR and Adenocarcinoma
 Heart Diseases Antibodies  >19 publications with EPOR and Heart Diseases

Organs/Tissues associated with EPOR cell lysate

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >295 publications with EPOR and Blood
 Bone Antibodies  >112 publications with EPOR and Bone
 Brain Antibodies  >92 publications with EPOR and Brain
 Kidney Antibodies  >61 publications with EPOR and Kidney
 Liver Antibodies  >60 publications with EPOR and Liver
 Heart Antibodies  >36 publications with EPOR and Heart
 Embryonic Tissue Antibodies  >28 publications with EPOR and Embryonic Tissue
 Lung Antibodies  >21 publications with EPOR and Lung
 Eye Antibodies  >20 publications with EPOR and Eye
 Skin Antibodies  >10 publications with EPOR and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000