• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

FABP4 Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8116764

Fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to thecalycin superfamily and Fatty-acid binding… More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FABP4, Cell Lysate
Full Product Name
Mouse FABP4 HEK293 Overexpression Lysate
Product Gene Name
FABP4 cell lysate
Product Synonym Names
Mouse FABP4/ALBP/A-FABP HEK293 Cell Lysate (WB positive control); fatty acid binding protein 4, adipocyte; Mouse 422/aP2 Overexpression Lysate; Mouse ALBP/Ap2 Overexpression Lysate; Mouse Ap2 Overexpression Lysate; Mouse Lbpl Overexpression Lysate
Sequence
Met1-Ala13 2
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
14,650 Da
Sequence Positions
143aa
3D Structure
ModBase 3D Structure for P04117
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse FABP4 (NP_077717.1) (Met 1-Ala 132) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Product Categories/Family for FABP4 cell lysate

Early Mesodermal Lineage Markers
Applications Tested/Suitable for FABP4 cell lysate
Western Blot (WB)
Application Notes for FABP4 cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for FABP4 cell lysate

van Dongen, M.J. et al., 2002, J Am Chem Soc. 124 (40): 11874-80. Coll, B. et al., 2008, Atherosclerosis 199 (1):147-53. Hoashi, S. et al., 2008, BMC Genet. 9 :84. Cai, H. et al., 2010, Bioorg Med Chem Lett 20 (12):3675-9.

Other Notes

Small volumes of FABP4 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FABP4 purchase

MBS8116764 is a cell lysate targeting FABP4. Also known as Mouse FABP4 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for FABP4. It may not necessarily be applicable to this product.

NCBI GI #
14149635
NCBI GeneID
11770
NCBI Accession #
NP_077717.1
UniProt Primary Accession #
P04117
UniProt Related Accession #
P04117
GenBank Nucleotide #
NM_024406.2
NCBI Official Full Name
fatty acid-binding protein, adipocyte
NCBI Official Synonym Full Names
fatty acid binding protein 4, adipocyte
NCBI Official Symbol
Fabp4
NCBI Official Synonym Symbols
Ap2; Lbpl; 422/aP2; ALBP/Ap2
UniProt Protein Name
Fatty acid-binding protein, adipocyte
UniProt Synonym Protein Names
3T3-L1 lipid-binding protein; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422
Protein Family
Fatty acid-binding protein
UniProt Gene Name
Fabp4
UniProt Synonym Gene Names
Ap2; ALBP; A-FABP; AFABP
UniProt Entry Name
FABP4_MOUSE
NCBI Protein Information
fatty acid-binding protein, adipocyte

UniProt Comments for FABP4

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm; cytosol

Biological Process: brown fat cell differentiation; cholesterol homeostasis; cytokine production; fatty acid metabolic process; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of inflammatory response; white fat cell differentiation
Show more

Research Articles on FABP4

1. Data suggest Fabp4 is up-regulated and DNA methylation is down-regulated in aorta in hyperhomocysteinemia induced by high-methionine diet; up-regulation of DNA methyltransferase 1 (Dnmt1) promotes DNA methylation and decreases Fabp4 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FABP4 cell lysate

 Reference Product  PubMed Publications
 Cebpa cell lysate  >115 publications with FABP4 and Cebpa
 Lpl cell lysate  >90 publications with FABP4 and Lpl
 Cd36 cell lysate  >57 publications with FABP4 and Cd36
 Adipoq cell lysate  >49 publications with FABP4 and Adipoq
 Ppargc1a cell lysate  >17 publications with FABP4 and Ppargc1a
 Wnt10b cell lysate  >14 publications with FABP4 and Wnt10b
 Plin1 cell lysate  >13 publications with FABP4 and Plin1
 Ppara cell lysate  >8 publications with FABP4 and Ppara
 Pck1 cell lysate  >2 publications with FABP4 and Pck1

Pathways associated with FABP4 cell lysate

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies  Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Mus Musculus Biological Processes Pathway antibodies  Mus Musculus Biological Processes Pathway Diagram
 PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway antibodies  PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram

Diseases associated with FABP4 cell lysate

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >179 publications with FABP4 and Insulin Resistance
 Inflammation Antibodies  >126 publications with FABP4 and Inflammation
 Cardiovascular Diseases Antibodies  >106 publications with FABP4 and Cardiovascular Diseases
 Fatty Liver Antibodies  >96 publications with FABP4 and Fatty Liver
 Necrosis Antibodies  >50 publications with FABP4 and Necrosis
 Heart Diseases Antibodies  >41 publications with FABP4 and Heart Diseases
 Liver Diseases Antibodies  >40 publications with FABP4 and Liver Diseases
 Disease Models, Animal Antibodies  >35 publications with FABP4 and Disease Models, Animal
 Weight Gain Antibodies  >29 publications with FABP4 and Weight Gain
 Weight Loss Antibodies  >25 publications with FABP4 and Weight Loss

Organs/Tissues associated with FABP4 cell lysate

 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >325 publications with FABP4 and Adipose Tissue
 Connective Tissue Antibodies  >215 publications with FABP4 and Connective Tissue
 Liver Antibodies  >113 publications with FABP4 and Liver
 Muscle Antibodies  >110 publications with FABP4 and Muscle
 Bone Marrow Antibodies  >68 publications with FABP4 and Bone Marrow
 Heart Antibodies  >66 publications with FABP4 and Heart
 Lung Antibodies  >26 publications with FABP4 and Lung
 Embryonic Tissue Antibodies  >26 publications with FABP4 and Embryonic Tissue
 Kidney Antibodies  >20 publications with FABP4 and Kidney
 Skin Antibodies  >14 publications with FABP4 and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000