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GDF15 Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8114240

Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-beta superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15/GDF15 is not expressed in the normal adult heart… More details

$315 / 0.3 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GDF15, Cell Lysate
Full Product Name
Human GDF15 HEK293 Overexpression Lysate
Product Gene Name
GDF15 cell lysate
Product Synonym Names
Human GDF-15 HEK293 Cell Lysate (WB positive control); growth differentiation factor 15; Human GDF-15 Overexpression Lysate; Human MIC-1 Overexpression Lysate; Human MIC1 Overexpression Lysate; Human NAG-1 Overexpression Lysate; Human PDF Overexpression Lysate; Human PLAB Overexpression Lysate; Human PTGFB Overexpression Lysate
Sequence
Ala197-Ile 308
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
150,000
Sequence Positions
370aa
Chromosome Location
Chromosome: 14; NC_000014.8 (106207810..106209407, complement). Location: 14q32.33
OMIM
147100
3D Structure
ModBase 3D Structure for P01857
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mature form of human GDF15 (P01857.1) (Ala 197-Ile 308) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for GDF15 cell lysate
Western Blot (WB)
Application Notes for GDF15 cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for GDF15 cell lysate

Ago T, et al. (2006) GDF15, a cardioprotective TGF-beta superfamily protein. Circ Res. 98 (3): 294-297.Hsiao E, et al. (2000) Characterization of growth-differentiation factor 15, a transforming growth factor beta superfamily member induced following liver injury. Mol Cell Biol. 20 (10): 3742-51.Zimmers T, et al. (2005) Growth differentiation factor-15/macrophage inhibitory cytokine-1 induction after kidney and lung injury. Shock. 23 (6): 543-8.

Other Notes

Small volumes of GDF15 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GDF15 purchase

MBS8114240 is a cell lysate targeting GDF15. Also known as Human GDF15 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GDF15. It may not necessarily be applicable to this product.

NCBI GI #
121039
NCBI GeneID
3500
NCBI Accession #
P01857.1
UniProt Primary Accession #
P01857
UniProt Related Accession #
P01857
NCBI Official Full Name
Ig gamma-1 chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Official Symbol
IGHG1
UniProt Protein Name
Ig gamma-1 chain C region
Protein Family
Growth/differentiation factor
UniProt Gene Name
IGHG1
UniProt Entry Name
IGHG1_HUMAN

UniProt Comments for GDF15

IGHG1: Defects in IGHG1 are a cause of multiple myeloma (MM). MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. A chromosomal aberration involving IGHG1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Protein type: Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; antigen binding

Biological Process: innate immune response; complement activation, classical pathway; complement activation

Disease: Myeloma, Multiple
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Research Articles on GDF15

1. The presence of IGHG1 in pancreatic cancer cells might constitute an important element responsible for tumor cell proliferation and immune evasion mechanisms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GDF15 cell lysate

 Reference Product  PubMed Publications
 IGKC cell lysate  >1 publications with GDF15 and IGKC

Pathways associated with GDF15 cell lysate

 Products by Pathway  Pathway Diagram
 Classical Antibody-mediated Complement Activation Pathway antibodies  Classical Antibody-mediated Complement Activation Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Creation Of C4 And C2 Activators Pathway antibodies  Creation Of C4 And C2 Activators Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway antibodies  Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway Diagram

Organs/Tissues associated with GDF15 cell lysate

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with GDF15 and Blood
 Lymph Antibodies  >2 publications with GDF15 and Lymph
 Lymph Node Antibodies  >2 publications with GDF15 and Lymph Node
 Intestine Antibodies  >2 publications with GDF15 and Intestine
 Skin Antibodies  >2 publications with GDF15 and Skin
 Bone Antibodies  >1 publications with GDF15 and Bone
 Bone Marrow Antibodies  >1 publications with GDF15 and Bone Marrow
 Thyroid Antibodies  >1 publications with GDF15 and Thyroid
 Pancreas Antibodies  >1 publications with GDF15 and Pancreas
 Uterus Antibodies  >1 publications with GDF15 and Uterus
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