GM2A Cell Lysate – Tissue Lysate

Name: GM2A Cell Lysate; Tissue Lysate; MyBioSource
Brand: MyBioSource
SKU: MBS8115100
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8115100

GM2A (GM2 ganglioside activator), is a lipid transfer protein which belongs to the ML domain family. GM2A can accommodate several single chain phospholipids and fatty acids. It also exhibits some calcium-independent phospholipase activity. GM2A binds… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GM2A, Cell Lysate

Full Product Name

Human GM2A Baculovirus-Insect cells Overexpression Lysate

Product Gene Name

GM2A cell lysate

Product Synonym Names

Human GM2A Insect Cell Lysate (WB positive control); GM2 ganglioside activator; Human GM2-AP Overexpression Lysate; Human SAP-3 Overexpression Lysate

Sequence

Met1-Ile19 3

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Molecular Weight

20,838 Da

Sequence Positions

180aa

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human GM2A (AAA35907.1) (Met 1-Ile 193) was fused with a polyhistidine tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Applications Tested/Suitable for GM2A cell lysate
Western Blot (WB)

Application Notes for GM2A cell lysate
WB: Use at an assay dependent dilution.

Related Product Information for GM2A cell lysate
GM2A (GM2 ganglioside activator), is a lipid transfer protein which belongs to the ML domain family. GM2A can accommodate several single chain phospholipids and fatty acids. It also exhibits some calcium-independent phospholipase activity. GM2A binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. GM2A acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Defects in GM2A are the cause of GM2-gangliosidosis type AB (GM2GAB), also known as Tay-Sachs disease AB variant.

This Human GM2A overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of GM2A protein from the overexpression lysate was verified.

Product References and Citations for GM2A cell lysate
Wright CS, et al. (2003) Structural analysis of lipid complexes of GM2-activator protein. J Mol Biol. 331(4):951-64.

Other Notes
Small volumes of GM2A cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GM2A purchase
MBS8115100 is a cell lysate targeting GM2A. Also known as Human GM2A Baculovirus-Insect cells Overexpression Lysate. Host: Baculovirus-Insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GM2A. It may not necessarily be applicable to this product.

NCBI GI #
183357
NCBI GeneID
2760
NCBI Accession #
AAA35907.1

UniProt Secondary Accession #
Q14426; Q14428; Q6LBL5; B2R699; D3DQH6
UniProt Related Accession #
P17900

NCBI Official Full Name
G-M2 activator protein
NCBI Official Synonym Full Names
GM2 ganglioside activator
NCBI Official Symbol
GM2A
NCBI Official Synonym Symbols
SAP-3; GM2-AP

Protein Family
Ganglioside GM2 activator

NCBI Protein Information
ganglioside GM2 activator

NCBI Summary for GM2A

This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
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Research Articles on GM2A

1. Studies indicate that sphingolipid activator proteins (SAPs) and anionic lipids are essential stimulators to reach physiological rates of lysosomal sphingolipid degradation.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GM2A cell lysate

Products by Pathway Pathway Diagram

Glycosphingolipid Metabolism Pathway antibodies Glycosphingolipid Metabolism Pathway Diagram

Immune System Pathway antibodies Immune System Pathway Diagram

Innate Immune System Pathway antibodies Innate Immune System Pathway Diagram

Lysosome Pathway antibodies Lysosome Pathway Diagram

Lysosome Pathway antibodies Lysosome Pathway Diagram

Metabolism Pathway antibodies Metabolism Pathway Diagram

Metabolism Of Lipids And Lipoproteins Pathway antibodies Metabolism Of Lipids And Lipoproteins Pathway Diagram

Neutrophil Degranulation Pathway antibodies Neutrophil Degranulation Pathway Diagram

Sphingolipid Metabolism Pathway antibodies Sphingolipid Metabolism Pathway Diagram

Products by Pathway	Pathway Diagram
Glycosphingolipid Metabolism Pathway antibodies	Glycosphingolipid Metabolism Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Neutrophil Degranulation Pathway antibodies	Neutrophil Degranulation Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram