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IGFBP2 Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8117064

IGFBP-2, also known as IGFBP2, is an insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs, control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF… More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IGFBP2, Cell Lysate
Full Product Name
Mouse IGFBP2 HEK293 Overexpression Lysate
Product Gene Name
IGFBP2 cell lysate
Product Synonym Names
Mouse IGFBP-2/IGFBP2 HEK293 Cell Lysate (WB positive control); insulin-like growth factor binding protein 2, 36kDa; Mouse AI255832 Overexpression Lysate; Mouse IBP-2 Overexpression Lysate; Mouse Igfbp-2 Overexpression Lysate; Mouse mIGFBP-2 Overexpression Lysate
Sequence
Met1-Gln30 5
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
32,847 Da
Sequence Positions
512aa
3D Structure
ModBase 3D Structure for P47877
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Igfbp2 (NP_032368.2) (Met1-Gln305) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for IGFBP2 cell lysate
Western Blot (WB)
Application Notes for IGFBP2 cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for IGFBP2 cell lysate

Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2). EMBO J. 8(9):2497-502.Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.

Other Notes

Small volumes of IGFBP2 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGFBP2 purchase

MBS8117064 is a cell lysate targeting IGFBP2. Also known as Mouse IGFBP2 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for IGFBP2. It may not necessarily be applicable to this product.

NCBI GI #
31982260
NCBI GeneID
16008
NCBI Accession #
NP_032368.2
UniProt Primary Accession #
P47877
UniProt Secondary Accession #
Q61722; Q91VK7
UniProt Related Accession #
P47877
GenBank Nucleotide #
NM_008342.3
NCBI Official Full Name
insulin-like growth factor-binding protein 2 isoform 1
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 2
NCBI Official Symbol
Igfbp2
NCBI Official Synonym Symbols
IBP-2; Igfbp-2; AI255832; mIGFBP-2
UniProt Protein Name
Insulin-like growth factor-binding protein 2
Protein Family
Insulin-like growth factor-binding protein
UniProt Gene Name
Igfbp2
UniProt Synonym Gene Names
Igfbp-2; IBP-2; IGF-binding protein 2; IGFBP-2; mIGFBP-2
UniProt Entry Name
IBP2_MOUSE
NCBI Protein Information
insulin-like growth factor-binding protein 2

NCBI Summary for IGFBP2

The protein encoded by this gene is one of several similar proteins that bind insulin-like growth factors I and II (Igf-I and Igf-II). The encoded protein can be secreted into the bloodstream, where it binds Igf-I and Igf-II with high affinity, or it can remain intracellular, interacting with many different ligands. Two transcript variants, one encoding a secreted isoform and the other encoding a nonsecreted isoform, have been found for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for IGFBP2

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; insulin-like growth factor II binding

Biological Process: regulation of insulin-like growth factor receptor signaling pathway; regulation of growth; regulation of cell growth; positive regulation of activated T cell proliferation
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Research Articles on IGFBP2

1. that the lacking the Arg-Gly-Asp-domain has a major influence on the pleiotropic effects of IGFBP-2 and suggest that somatic growth and time of sexual maturity or somatic growth and life expectancy are less closely related than thought previously

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IGFBP2 cell lysate

 Reference Product  PubMed Publications
 Igf1 cell lysate  >67 publications with IGFBP2 and Igf1
 Igf2 cell lysate  >54 publications with IGFBP2 and Igf2
 Pten cell lysate  >15 publications with IGFBP2 and Pten
 Trp53 cell lysate  >4 publications with IGFBP2 and Trp53
 Lep cell lysate  >1 publications with IGFBP2 and Lep
 Fgf2 cell lysate  >1 publications with IGFBP2 and Fgf2
 Miip cell lysate  >1 publications with IGFBP2 and Miip
 Ins2 cell lysate  >1 publications with IGFBP2 and Ins2

Pathways associated with IGFBP2 cell lysate

 Products by Pathway  Pathway Diagram
 Factors And Pathways Affecting Insulin-like Growth Factor (IGF1)-Akt Signaling antibodies  Factors And Pathways Affecting Insulin-like Growth Factor (IGF1)-Akt Signaling Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with IGFBP2 cell lysate

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >24 publications with IGFBP2 and Insulin Resistance
 Adenocarcinoma Antibodies  >23 publications with IGFBP2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >21 publications with IGFBP2 and Breast Neoplasms
 Obesity Antibodies  >19 publications with IGFBP2 and Obesity
 Disease Models, Animal Antibodies  >13 publications with IGFBP2 and Disease Models, Animal
 Necrosis Antibodies  >12 publications with IGFBP2 and Necrosis
 Neoplasms, Experimental Antibodies  >11 publications with IGFBP2 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >11 publications with IGFBP2 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >10 publications with IGFBP2 and Inflammation
 Cardiovascular Diseases Antibodies  >10 publications with IGFBP2 and Cardiovascular Diseases

Organs/Tissues associated with IGFBP2 cell lysate

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >62 publications with IGFBP2 and Brain
 Liver Antibodies  >40 publications with IGFBP2 and Liver
 Bone Antibodies  >35 publications with IGFBP2 and Bone
 Muscle Antibodies  >28 publications with IGFBP2 and Muscle
 Ovary Antibodies  >17 publications with IGFBP2 and Ovary
 Lung Antibodies  >15 publications with IGFBP2 and Lung
 Kidney Antibodies  >11 publications with IGFBP2 and Kidney
 Embryonic Tissue Antibodies  >10 publications with IGFBP2 and Embryonic Tissue
 Eye Antibodies  >9 publications with IGFBP2 and Eye
 Mammary Gland Antibodies  >6 publications with IGFBP2 and Mammary Gland
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