IGFL2 Cell Lysate – Tissue Lysate

Name: IGFL2 Cell Lysate; Tissue Lysate; MyBioSource
Brand: MyBioSource
SKU: MBS8115204
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8115204

This Human IGFL2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IGFL2 protein from the overexpression lysate was verified. More details

$315 / 0.3 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IGFL2, Cell Lysate

Full Product Name

Human IGFL2 HEK293 Overexpression Lysate

Product Gene Name

IGFL2 cell lysate

Product Synonym Names

Human IGFL2 HEK293 Cell Lysate (WB positive control); IGF like family member 2; Human IGFL2 Overexpression Lysate; Human UNQ645 Overexpression Lysate; Human VPRI645 Overexpression Lysate

Sequence

Pro26-Pro1 19

Host

HEK293 Cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Molecular Weight

14,489 Da

Sequence Positions

354aa

OMIM

610545

3D Structure

ModBase 3D Structure for Q6UWQ7

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human IGFL2 (NP_001002915.2) (Pro26-Pro119) was expressed with the Fc region of human IgG1 at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Applications Tested/Suitable for IGFL2 cell lysate
Western Blot (WB)

Application Notes for IGFL2 cell lysate
WB: Use at an assay dependent dilution.

Related Product Information for IGFL2 cell lysate
This Human IGFL2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IGFL2 protein from the overexpression lysate was verified.

Other Notes
Small volumes of IGFL2 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGFL2 purchase
MBS8115204 is a cell lysate targeting IGFL2. Also known as Human IGFL2 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for IGFL2. It may not necessarily be applicable to this product.

NCBI GI #
205361122
NCBI GeneID
147920
NCBI Accession #
NP_001002915.2

UniProt Primary Accession #
Q6UWQ7
UniProt Secondary Accession #
Q6B9Z3; E9PAV1
UniProt Related Accession #
Q6UWQ7
GenBank Nucleotide #
NM_001002915.2

NCBI Official Full Name
insulin growth factor-like family member 2 isoform a
NCBI Official Synonym Full Names
IGF-like family member 2
NCBI Official Symbol
IGFL2
NCBI Official Synonym Symbols
UNQ645; VPRI645

UniProt Protein Name
Insulin growth factor-like family member 2
Protein Family
Insulin growth factor-like family
UniProt Gene Name
IGFL2
UniProt Entry Name
IGFL2_HUMAN

NCBI Protein Information
insulin growth factor-like family member 2

NCBI Summary for IGFL2

IGFL2 belongs to the insulin-like growth factor (IGF; see MIM 147440) family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development, especially prenatal growth (Emtage et al., 2006 [PubMed 16890402]).[supplied by OMIM, Mar 2008]

UniProt Comments for IGFL2

IGFL2: Potential ligand of the IGFLR1 cell membrane receptor. Belongs to the IGFL family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.