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PFKP Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8115441

PFKP plays a critical role in cell proliferation in breast cancer cells. PFKP is necessary for starvation-mediated autophagy, glycolysis, and EMT, thereby promoting the malignant progression of OSCC. The Snail-PFKP axis plays an important role in cancer cell… More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PFKP, Cell Lysate
Full Product Name
Human PFKP Baculovirus-Insect Overexpression Lysate
Product Gene Name
PFKP cell lysate
Product Synonym Names
Human PFKP Insect Cell Lysate (WB positive control); phosphofructokinase, platelet; Human ATP-PFK Overexpression Lysate; Human PFK-C Overexpression Lysate; Human PFK-P Overexpression Lysate; Human PFKF Overexpression Lysate
Sequence
His-GST
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
85,316 Da
Sequence Positions
1021aa
OMIM
171840
3D Structure
ModBase 3D Structure for Q01813
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Sequence Construction
A DNA sequence encoding the human PFKP (NP_002618.1) (Met1-Val784) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST).
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for PFKP cell lysate
Western Blot (WB)
Application Notes for PFKP cell lysate
WB: Use at an assay dependent dilution.

Other Notes

Small volumes of PFKP cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKP purchase

MBS8115441 is a cell lysate targeting PFKP. Also known as Human PFKP Baculovirus-Insect Overexpression Lysate. Host: Baculovirus-Insect Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKP. It may not necessarily be applicable to this product.

NCBI GI #
11321601
NCBI GeneID
5214
NCBI Accession #
NP_002618.1
UniProt Primary Accession #
Q01813
UniProt Secondary Accession #
Q5VSR7; Q5VSR8; B3KS15
UniProt Related Accession #
Q01813
GenBank Nucleotide #
NM_002627.4
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, platelet type isoform 1
NCBI Official Synonym Full Names
phosphofructokinase, platelet
NCBI Official Symbol
PFKP
NCBI Official Synonym Symbols
PFKF; PFK-C; PFK-P; ATP-PFK
UniProt Protein Name
6-phosphofructokinase type C
UniProt Synonym Protein Names
6-phosphofructokinase, platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase
Protein Family
ATP-dependent 6-phosphofructokinase
UniProt Gene Name
PFKP
UniProt Synonym Gene Names
PFKF; PFK-C
UniProt Entry Name
K6PP_HUMAN
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; phosphohexokinase; phosphofructokinase 1; 6-phosphofructokinase type C; phosphofructo-1-kinase isozyme C; Phosphofructokinase, platelet type

NCBI Summary for PFKP

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]
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UniProt Comments for PFKP

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Kinase, other; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: metal ion binding; protein complex binding; 6-phosphofructokinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process
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Research Articles on PFKP

1. Anti-human protein S antibody induces tissue factor expression through a direct interaction with PFKP and ERK1/2 activation in coronary artery endothelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PFKP cell lysate

 Reference Product  PubMed Publications
 ALDOA cell lysate  >4 publications with PFKP and ALDOA
 HK2 cell lysate  >2 publications with PFKP and HK2
 HK1 cell lysate  >2 publications with PFKP and HK1
 PFKFB3 cell lysate  >1 publications with PFKP and PFKFB3
 GPI cell lysate  >1 publications with PFKP and GPI

Pathways associated with PFKP cell lysate

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram

Diseases associated with PFKP cell lysate

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with PFKP and Disease Models, Animal
 Adenocarcinoma Antibodies  >1 publications with PFKP and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >1 publications with PFKP and Neoplasms, Experimental
 Hypertension Antibodies  >1 publications with PFKP and Hypertension
 Weight Gain Antibodies  >1 publications with PFKP and Weight Gain
 Breast Neoplasms Antibodies  >1 publications with PFKP and Breast Neoplasms
 Liver Diseases Antibodies  >1 publications with PFKP and Liver Diseases
 Liver Neoplasms Antibodies  >1 publications with PFKP and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with PFKP and Carcinoma, Hepatocellular
 Kidney Diseases Antibodies  >1 publications with PFKP and Kidney Diseases

Organs/Tissues associated with PFKP cell lysate

 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >6 publications with PFKP and Ovary
 Blood Antibodies  >6 publications with PFKP and Blood
 Liver Antibodies  >4 publications with PFKP and Liver
 Muscle Antibodies  >4 publications with PFKP and Muscle
 Bone Antibodies  >2 publications with PFKP and Bone
 Thyroid Antibodies  >2 publications with PFKP and Thyroid
 Brain Antibodies  >2 publications with PFKP and Brain
 Vascular Antibodies  >1 publications with PFKP and Vascular
 Skin Antibodies  >1 publications with PFKP and Skin
 Kidney Antibodies  >1 publications with PFKP and Kidney
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