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PGF Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8116397

This Mouse PLGF/PGF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PLGF/PGF protein from the overexpression lysate was verified. More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLGF/PGF, Cell Lysate
Full Product Name
Mouse PLGF/PGF HEK293 Overexpression Lysate
Product Gene Name
PGF cell lysate
Product Synonym Names
Mouse PIGF/PLGF HEK293 Cell Lysate (WB positive control); placental growth factor; Mouse AI854365 Overexpression Lysate; Mouse PIGF Overexpression Lysate; Mouse Plgf Overexpression Lysate
Sequence
Met1-Pro15 8
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
17,876 Da
Sequence Positions
381aa
3D Structure
ModBase 3D Structure for P49764
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse PGF (P49764) (Met 1-Pro 158) was fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for PGF cell lysate
Western Blot (WB)
Application Notes for PGF cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for PGF cell lysate

Nagy JA, et al. (2003) VEGF-A(164/165) and PLGF: roles in angiogenesis and arteriogenesis. Trends Cardiovasc Med. 13(5): 169-75.Chaballe L, et al. (2011) Placental growth factor: a tissue modelling factor with therapeutic potentials in neurology? Acta Neurol Belg. 111(1): 10-7.Odorisio T, et al. (2006) The placental growth factor in skin angiogenesis. J Dermatol Sci. 41(1): 11-9.

Other Notes

Small volumes of PGF cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGF purchase

MBS8116397 is a cell lysate targeting PGF. Also known as Mouse PLGF/PGF HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PGF. It may not necessarily be applicable to this product.

NCBI GI #
6679289
NCBI GeneID
18654
NCBI Accession #
NP_032853.1
UniProt Primary Accession #
P49764
UniProt Related Accession #
P49764
GenBank Nucleotide #
NM_008827.3
NCBI Official Full Name
placenta growth factor isoform 1
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
Pgf
NCBI Official Synonym Symbols
PIGF; Plgf; AI854365
UniProt Protein Name
Placenta growth factor
Protein Family
Placenta growth factor
UniProt Gene Name
Pgf
UniProt Synonym Gene Names
Plgf; PlGF
UniProt Entry Name
PLGF_MOUSE
NCBI Protein Information
placenta growth factor

UniProt Comments for PGF

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space

Molecular Function: growth factor activity; protein heterodimerization activity; protein homodimerization activity; vascular endothelial growth factor receptor binding

Biological Process: angiogenesis; induction of positive chemotaxis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; response to hypoxia; sprouting angiogenesis; ureteric bud branching; vascular endothelial growth factor receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PGF cell lysate

 Reference Product  PubMed Publications
 Flt1 cell lysate  >262 publications with PGF and Flt1
 Vegfa cell lysate  >139 publications with PGF and Vegfa
 Kdr cell lysate  >59 publications with PGF and Kdr
 Vegfc cell lysate  >26 publications with PGF and Vegfc
 Vegfb cell lysate  >12 publications with PGF and Vegfb
 Flt4 cell lysate  >9 publications with PGF and Flt4
 Tek cell lysate  >4 publications with PGF and Tek
 Pdgfrb cell lysate  >4 publications with PGF and Pdgfrb
 Figf cell lysate  >3 publications with PGF and Figf
 Pdgfra cell lysate  >1 publications with PGF and Pdgfra

Pathways associated with PGF cell lysate

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Ras Signaling Pathway antibodies  Ras Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with PGF cell lysate

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >399 publications with PGF and Cardiovascular Diseases
 Inflammation Antibodies  >299 publications with PGF and Inflammation
 Hypertension Antibodies  >199 publications with PGF and Hypertension
 Disease Models, Animal Antibodies  >167 publications with PGF and Disease Models, Animal
 Necrosis Antibodies  >162 publications with PGF and Necrosis
 Lung Diseases Antibodies  >133 publications with PGF and Lung Diseases
 Heart Diseases Antibodies  >132 publications with PGF and Heart Diseases
 Atherosclerosis Antibodies  >94 publications with PGF and Atherosclerosis
 Brain Diseases Antibodies  >74 publications with PGF and Brain Diseases
 Kidney Diseases Antibodies  >73 publications with PGF and Kidney Diseases

Organs/Tissues associated with PGF cell lysate

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >242 publications with PGF and Lung
 Embryonic Tissue Antibodies  >203 publications with PGF and Embryonic Tissue
 Brain Antibodies  >199 publications with PGF and Brain
 Liver Antibodies  >142 publications with PGF and Liver
 Eye Antibodies  >86 publications with PGF and Eye
 Stomach Antibodies  >71 publications with PGF and Stomach
 Intestine Antibodies  >62 publications with PGF and Intestine
 Extraembryonic Tissue Antibodies  >32 publications with PGF and Extraembryonic Tissue
 Pituitary Gland Antibodies  >32 publications with PGF and Pituitary Gland
 Spinal Cord Antibodies  >28 publications with PGF and Spinal Cord
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